AI Article Synopsis
- Cyclin E facilitates the formation of the pre-replication complex (pre-RC), while cyclin A is involved in activating DNA synthesis.
- Cyclin E is specifically localized to the nuclear matrix in differentiated vertebrate cells, but not in undifferentiated or cancer cells.
- As cells differentiate, cyclin E is recruited to the nuclear matrix, indicating that pre-RC assembly may become spatially organized, with lack of restriction potentially contributing to the flexibility seen in cancer cells.
Article Abstract
Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3074132 | PMC |
| http://dx.doi.org/10.1093/nar/gkq1190 | DOI Listing |
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