Macro-EMG records electrical activity from the whole motor unit (MU) as a parameter of its size. The main component of the recording device is a modified single fiber or concentric needle electrode. Different sized uptake areas allow to record with different selectivities from the MU. The teflon insulated shaft surface allows to record with a defined large blank area unselectively macro-signals from the muscle. The conceptual ideas to use a concentric needle in addition to the single fibre electrode is to simplify the trigger-potential recording, which needs to be selective within the MU. In both instances the EMG apparatus has to provide two channels, one for the trigger and the second for an averaging device to record the macro-EMG. The different techniques use the single fiber or concentric EMG-potential as a trigger to average the associated macro-potential from the raw signals. The electrodes different design allow the whole single-fiber-needle to lie within the MU territory whereas the MU is only touched by the concentric needles tip. This implies different recording situations and results. Anatomically macro-EMG size is varied by the recording site of the muscle. The signal is greater close to the endplate zone and declines to stable values about 20 mm apart. For quantitative measurements within a muscle 20 different potentials have to be evaluated for median amplitude, duration and area.
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Nat Commun
January 2025
State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, Anyang, China.
Cotton fibers are single cells that develop from the epidermal cells in the outer integument of developing seeds. The processes regulating fiber cell development have been extensively studied; however, the spatiotemporal transcriptome and metabolome profiles during the early stages of fiber development remain largely unknown. In this study, we profile the dynamics of transcriptome and metabolome during the early stages of cotton fiber cell development using a combination of spatial transcriptomic, single-cell transcriptomic, and spatial metabolomic analyses.
View Article and Find Full Text PDFFASEB J
January 2025
Department of Neurology, Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Skeletal muscle function gradually declines with aging, presenting substantial health and societal challenges. Comparative analysis of how aging affects fast- and slow-twitch muscles remains lacking. We utilized 20-month-old mice to reveal the aging effects on muscle structure and fiber composition, followed by bulk RNA sequencing for fast- and slow-twitch muscles and integration with human single-cell RNA sequencing dataset providing a comparative analysis across species.
View Article and Find Full Text PDFThe DNA damage response (DDR) mechanisms that allow cells to tolerate DNA replication stress are critically important for genome stability and cell viability. Using an unbiased genetic screen we identify a role for the RING finger E3 ubiquitin ligase RNF25 in promoting DNA replication stress tolerance. In response to DNA replication stress, RNF25-deficient cells generate aberrantly high levels of single-stranded DNA (ssDNA), accumulate in S-phase and show reduced mitotic entry.
View Article and Find Full Text PDFRNA-binding proteins (RBPs) are critical regulators of mRNAs controlling all processes such as RNA transcription, transport, localization, translation, mRNA:ncRNA interactions, and decay. Cellular differentiation is driven by tissue-specific and/or tissue-preferred expression of proteins needed for the optimal function of mature cells, tissues and organs. Lens fiber cell differentiation is marked by high levels of expression of crystallin genes encoding critical proteins for lens transparency and light refraction.
View Article and Find Full Text PDFPhotodiagnosis Photodyn Ther
January 2025
Department of Prosthetic Dental Sciences, College of Dentistry. King Saud University. Riyadh, Saudi Arabia PO Box-60169. Electronic address:
Aim: To investigate the effectiveness of unconventional post space disinfection techniques, specifically Er:YAG laser, Temoporfin, and Carbon Nanoparticles (CNPs), in improving Martens hardness (MH), eliminating the smear layer (SL), and enhancing the push-out bond strength (PBS) of glass fiber posts in canal dentin.
Methods: An in vitro study was conducted utilizing 80 single-rooted extracted human teeth, which were decoronated and subjected to standardized root canal preparation. The teeth were equally distributed into four experimental groups: Group 1 (NaOCl + EDTA), Group 2 (Er: YAG laser + EDTA), Group 3 (Temoporfin + EDTA), and Group 4 (CNPs + EDTA).
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