AI Article Synopsis
- Flavonoids are plant compounds that help protect plants from various stresses and are found in fenugreek seeds and stems as glycosides and isoflavones.
- Researchers cloned a cDNA for a chalcone isomerase named TFGCHI-1 from fenugreek leaves, which converts chalcones to flavanones in the lab.
- When introduced into an Arabidopsis mutant lacking this enzyme, TFGCHI-1 successfully restored function, resulting in increased flavonol glycoside levels compared to the wild type.
Article Abstract
Flavonoids belong to a group of plant natural products with variable phenolic structures and play important roles in protection against biotic and abiotic stress. Fenugreek (Trigonella foenum-graecum L.) seeds and stems contain flavonol glycosides and isoflavone derivatives. Up to now, the molecular features of fenugreek flavonoid biosynthesis have not been characterized. Here we present cloning of a cDNA encoding a chalcone isomerase (namely TFGCHI-1) from the leaves of T. foenum-graecum which convert chalcones to flavanones in vitro. Transformation of Arabidopsis loss-of-function TT5 (CHI) mutant with a TFGCHI-1 cDNA complemented TT5 and produced higher levels of flavonol glycosides than wild-type Col-0.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1055/s-0030-1250566 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Optimization of Ultraviolet-B Treatment for Enrichment of Total Flavonoids in Buckwheat Sprouts Using Response Surface Methodology and Study on Its Metabolic Mechanism.
Foods
December 2024
College of Food Science and Engineering, Yangzhou University, Yangzhou 210095, China.
Buckwheat possesses significant nutritional content and contains different bioactive compounds, such as total flavonoids, which enhance its appeal to consumers. This study employed single-factor experiments and the response surface methodology to identify the optimal germination conditions for enhancing the total flavonoid content in buckwheat sprouts through ultraviolet-B treatment. The research showed that buckwheat sprouts germinated for 3 days at a temperature of 28.
View Article and Find Full Text PDFEvolutionary analysis of anthocyanin biosynthetic genes: insights into abiotic stress adaptation.
Plant Mol Biol
December 2024
Research Center of Crop Stresses Resistance Technologies/ MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River, Yangtze University, Jingzhou, 434025, China.
Anthocyanin regulation can be fruitfully explored from a diverse perspective by studying distantly related model organisms. Land plants pioneers faced a huge evolutionary leap, involving substantial physiological and genetic changes. Anthocyanins have evolved alongside these changes, becoming versatile compounds capable of mitigating terrestrial challenges such as drought, salinity, extreme temperatures and high radiation.
View Article and Find Full Text PDFOverexpression Analysis of Suggests Its Involvement in Salt Tolerance and Flavonoid Pathway in .
Int J Mol Sci
November 2024
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin 150040, China.
Soil salinization is a significant environmental stress factor, threatening global agricultural yield and ecological security. Plants must effectively cope with the adverse effects of salt stress on survival and successful reproduction. Lateral Organ Boundaries (LOB) Domain (LBD) genes, a gene family encoding plant-specific transcription factors (TFs), play important roles in plant growth and development.
View Article and Find Full Text PDFMetabolomics and proteomics analyses of Chrysanthemi Flos: a mechanism study of changes in proteins and metabolites by processing methods.
Chin Med
November 2024
School of Pharmacy, Anhui University of Chinese Medicine, 350 Shaoquan Rd, Hefei, 230012, People's Republic of China.
Background: Chrysanthemi Flos is a traditional Chinese medicine with a long history of medicinal use. Prior research suggests that the intrinsic composition of Chrysanthemi Flos is affected by shade-drying and oven-drying methods. Nevertheless, the effects of these methods on the proteins and metabolites of Chrysanthemi Flos have not been extensively studied.
View Article and Find Full Text PDFMetabolic engineering of ADP1 for naringenin production.
Metab Eng Commun
December 2024
Faculty of Engineering and Natural Sciences, Tampere University, Hervanta Campus, 33720, Tampere, Finland.
Naringenin, a flavanone and a precursor for a variety of flavonoids, has potential applications in the health and pharmaceutical sectors. The biological production of naringenin using genetically engineered microbes is considered as a promising strategy. The naringenin synthesis pathway involving chalcone synthase (CHS) and chalcone isomerase (CHI) relies on the efficient supply of key substrates, malonyl-CoA and -coumaroyl-CoA.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!