Previous reports indicated that AtMYB103 has an important role in tapetum development, callose dissolution, and exine formation in A. thaliana anthers. Here, we further characterized its function in anther development by expression pattern analysis, transmission electron microscopy observation of the knockout mutant, and microarray analysis of downstream genes. A total of 818 genes differentially expressed between ms188 and the wild-type were identified by global expression profiling analysis. Functional classification showed that loss-of-function of AtMYB103 impairs cell wall modification, lipid metabolic pathways, and signal transduction throughout anther development. RNA in situ hybridization confirmed that transcription factors acting downstream of AtMYB103 (At1g06280 and At1g02040) were expressed in the tapetum and microspores at later stages, suggesting that they might have important roles in microsporogenesis. These results indicated that AtMYB103 is a crucial regulator of Arabidopsis anther development.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11427-010-4060-y | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Expression divergence of BAG gene family in maize under heat stress.
BMC Plant Biol
January 2025
Maize and Millet Research Institute, Yousafwala, Sahiwal, Pakistan.
Heat stress poses a significant challenge for maize production, especially during the spring when high temperatures disrupt cellular processes, impeding plant growth and development. The B-cell lymphoma-2 (Bcl-2) associated athanogene (BAG) gene family is known to be relatively conserved across various species. It plays a crucial role as molecular chaperone cofactors that are responsible for programmed cell death and tumorigenesis.
View Article and Find Full Text PDFCRISPR/Cas9-mediated knockout of GhAMS11 and GhMS188 reveals key roles in tapetal development and pollen exine formation in upland cotton.
Int J Biol Macromol
December 2024
National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China. Electronic address:
The ABORTED MICROSPORES (AMS) gene is crucial for tapetal cell development and pollen formation, but its role in Upland cotton (Gossypium hirsutum) has not been previously documented. This study identified GhAMS11 as a key transcription factor, with its high expression specifically observed during the S4-S6 stages of anther development, a critical period for tapetal activity and pollen formation. Subcellular localization confirmed that GhAMS11 was located in the nucleus.
View Article and Find Full Text PDFTranscriptomics and metabolomics analyses reveal pollen abortion mechanism in alfalfa early stage male sterile lines.
Front Plant Sci
December 2024
Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences (JAAS), Changchun, China.
Alfalfa ( L.), a prominent perennial forage in the legume family, is widely cultivated across Europe and America. Given its substantial economic value for livestock, breeding efforts have focused on developing high-yield and high-quality varieties since the discovery of CMS lines.
View Article and Find Full Text PDFPlastid-localized ZmENR1/ZmHAD1 complex ensures maize pollen and anther development through regulating lipid and ROS metabolism.
Nat Commun
December 2024
Research Institute of Biology and Agriculture, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Lipid metabolism is critical for male reproduction in plants. Many lipid-metabolic genic male-sterility (GMS) genes function in the anther tapetal endoplasmic reticulum, while little is known about GMS genes involved in de novo fatty acid biosynthesis in the anther tapetal plastid. In this study, we identify a maize male-sterile mutant, enr1, with early tapetal degradation, defective anther cuticle, and pollen exine.
View Article and Find Full Text PDFThe regulatory module BnamiR827-BnaA09.NLA1-BnaPHT1s modulates phosphate homeostasis, pollen viability and seed yield in Brassica napus.
J Exp Bot
December 2024
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
Phosphorus (P) is an essential macronutrient for the growth and yield of crops. However, there is limited understanding of the regulatory mechanisms of phosphate (Pi) homeostasis, and its impact on growth, development, and yield-related traits in Brassica napus. Here, we identified four NITROGEN LIMITATION ADAPATATION1 (BnaNLA1) genes in B.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!