Blood cell capture on antibody microarrays and monitoring of the cell capture using surface plasmon resonance imaging.

Methods Mol Biol

CREAB Group, SPrAM-UMR 5819 (CEA-CNRS-UJF), INAC/CEA-Grenoble, Grenoble, France.

Published: March 2011

Blood is a tremendous source of data for diagnostic purposes. Thanks to the qualitative and quantitative analysis of the different cell types carried into the blood stream. To that end, cell capture of several cell types at different locations on a microarray is an interesting alternative to classical techniques run 'in solution' as it allows simultaneous characterization of several cells on a single device. In this chapter, we have described a method based on the production of antibody microarrays specific to two different cell types: B and T lymphocytes. We have also described the real-time monitoring of the cell capture on the microarray using surface plasmon resonance imaging (SPRi).

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http://dx.doi.org/10.1007/978-1-61737-970-3_11DOI Listing

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