Objective: To explore the effect and the intracellular signal transduction pathway of insulin-like growth factor 1 (IGF-1) on the expression of stem cell factor (SCF) in gastric smooth muscle cells (SMC).
Methods: Gastric SMC from SD rats were cultured by enzymolysis and identified by α-actin immunofluorescence methods. Western blot and quantitative reverse transcription-polymerase chain reaction were used to examine the expression of SCF in gastric SMC:(1) The level of SCF after gastric SMC were cultured with IGF-1. (2) The level of SCF after IGF-1 receptor (IGF-1Rα) monoclonal antibody were added. (3) Another SMC were pretreated with specific mitogen-activated protein kinase (MEK) inhibitor PD-98059 and phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor LY-294002, and investigate expression of SCF in gastric SMC.
Results: A very low level of SCF was expressed in gastric SMC cultured in bovine serum free medium. A low concentration of IGF-1 (5 and 10 µg/L) had no effect on the expression of SCF (both P>0.05), but the expressions of SCF mRNA and protein increased in IGF-1 at a higher concentration (50, 100 and 150 µg/L) (2.79, 5.51 and 5.35-fold in protein respectively, 1.81, 2.54 and 2.38-fold in mRNA respectively, all P<0.05), and IGF-1 in 100 µg/L may be the effective final concentration (all P<0.05). The peak of SCF increment was at the 16th hour with IGF-1 (2.36-fold in protein, 5.51-fold in mRNA, all P<0.05). The expression of SCF could be inhibited by IGF-1 receptor monoclonal antibody in a dose-dependent manner (all P<0.05). The IGF-1-induced SCF expression was reduced significantly by a pretreatment of PD-98059 (23% in protein and 48% in mRNA, P<0.05). And LY-294002 had no effect on the expression of SCF (P>0.05).
Conclusion: The SCF expression in gastric SMC is stimulated by IGF-1 in both dose- and time-dependent manners through IGF-1R in which ERKMAPK signal transduction may play an important role.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Effects of BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats.
World J Gastroenterol
January 2025
Department of Gastroenterology, The Air Force Medical Center, Beijing 100142, China.
Background: Simulated microgravity environment can lead to gastrointestinal motility disturbance. The pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor (SCF)/c-kit signaling pathway associated with intestinal flora and Cajal stromal cells. Moreover, intestinal flora can also affect the regulation of SCF/c-kit signaling pathway, thus affecting the expression of Cajal stromal cells.
View Article and Find Full Text PDFThymoquinone Pectin Beads Produced via Electrospray: Enhancing Oral Targeted Delivery for Colorectal Cancer Therapy.
Pharmaceutics
November 2024
Faculty of Pharmacy, Universiti Sultan Zainal Abidin, Besut Campus, Besut 22200, Malaysia.
: Thymoquinone (TQ) exhibits diverse biological activities, but its poor solubility and bioavailability limit its cancer efficacy, requiring innovative solutions. This study explores the development of an oral delivery system targeting colon cancer based on TQ pectin beads (TQ-PBs) produced through an adjustable electrospray technique. This study hypothesised that adjusting bead diameter through the electrospray technique enables precise control over water absorption and erosion rates, thereby achieving a controlled release profile for encapsulated TQ, which enhances targeted delivery to the colon.
View Article and Find Full Text PDFHesperidin facilitating gastrointestinal motility by "Gut-brain axis" and "SCF/C-Kit signaling pathways".
Poult Sci
December 2024
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Changchun 130118, China; Jilin Key Laboratory of Animal Nutrition and Feed Sciene, Jilin Agricultural University, Changchun 130118, China.
Hesperidin shows promising results as a potential feed additive for enhancing gastrointestinal motility in animals. Gastrointestinal function plays a pivotal role in animal growth and the digestibility of dietary nutrients, with gastrointestinal motor function serving as a crucial component. However, limited research has been conducted on the application of hesperidin as a feed additive to promote gastrointestinal motility.
View Article and Find Full Text PDFRapid identification of primary atopic disorders (PAD) by a clinical landmark-guided, upfront use of genomic sequencing.
Allergol Select
October 2024
Center for Child and Adolescent Health, Helios Hospital Krefeld, Academic Hospital of RWTH Aachen, Krefeld.
Article Synopsis
- Primary atopic disorders (PAD) are rare genetic conditions caused by specific gene variants that affect skin and immune function, making diagnosis challenging among common allergic disease cases.
- Identifying PAD requires recognizing clinical red flags like family history and unusual infections, as conventional lab tests are inadequate for definitive diagnosis.
- Whole-genome sequencing (WGS) enhances diagnostic efficiency and accuracy, but requires careful interpretation and collaboration among specialists to effectively manage PAD cases.
Naringenin Promotes Gastrointestinal Motility in Mice by Impacting the SCF/c-Kit Pathway and Gut Microbiota.
Foods
August 2024
College of Life Sciences, Shanxi Agricultural University, Taigu 030801, China.
Article Synopsis
- * The study discovered that NRG increases gastrointestinal hormone release, enhancing levels of gastrin and motilin while decreasing vasoactive intestinal peptide, which suggests how it improves digestive processes.
- * NRG appears to work by boosting the expression of certain proteins in the stomach and intestines, leading to a healthier balance of gut bacteria, promoting beneficial strains while reducing harmful ones.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!