In the S2 cell system of Drosophila melanogaster a key protein domain mediating the interaction of TFIID and Brahma transcriptional complexes into the BTFly supercomplex has been shown to be an evolutionary conserved SAY domain of the SAYP. TFIID and Brahma coactivators participated in the reporter gene activation induced by the SAY domain in cellular nuclei. The TFIID and Brahma components directly interacting with the SAY domain were identified.
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