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The effect of the pandemic on autoantibody rates in the general population.
Arch Rheumatol
December 2024
Department of Medical Microbiology, Division of Medical Virology, Necmettin Erbakan University Meram Faculty of Medicine, Konya, Türkiye.
Objectives: The study aimed to investigate the possible effects of coronavirus disease 2019 (COVID-19) on autoantibodies.
Patients And Methods: Samples of 89,108 individuals (29,033 males, 60,075 females; median: 36 years; range, 0 to 96 years) who underwent autoimmune testing between January 2017 and May 2022 were retrospectively analyzed. The prepandemic period was defined as May 1, 2017, to March 20, 2020, while the pandemic period was defined as March 20, 2020, to May 31, 2022.
Packaged delivery of CRISPR-Cas9 ribonucleoproteins accelerates genome editing.
Nucleic Acids Res
February 2025
Department of Chemistry, University of California, Berkeley, CA 94720, United States.
Effective genome editing requires a sufficient dose of CRISPR-Cas9 ribonucleoproteins (RNPs) to enter the target cell while minimizing immune responses, off-target editing, and cytotoxicity. Clinical use of Cas9 RNPs currently entails electroporation into cells ex vivo, but no systematic comparison of this method to packaged RNP delivery has been made. Here we compared two delivery strategies, electroporation and enveloped delivery vehicles (EDVs), to investigate the Cas9 dosage requirements for genome editing.
View Article and Find Full Text PDFThe G3BP1 RNP complex at focal adhesion tunes cell migration.
Trends Cell Biol
March 2025
School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China. Electronic address:
Ribonucleoprotein (RNP) complexes can form multiple mesoscale assemblies, including stress granules (SGs). However, the function and regulation of the soluble RNP complexes are not fully understood. A recent study by Boraas et al.
View Article and Find Full Text PDFSimultaneous gene editing of both nuclei in a dikaryotic strain of Ganoderma lucidum using Cas9-gRNA ribonucleoprotein.
J Microbiol
January 2025
Department of BioMedical Bigdata (BK21) and Research Institute of Life Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea.
The presence of multiple nuclei in a common cytoplasm poses a significant challenge to genetic modification in mushrooms. Here, we demonstrate successful gene editing in both nuclei of a dikaryotic strain of Ganoderma lucidum using the Cas9-gRNA ribonucleoprotein complex (RNP). The RNP targeting the pyrG gene was introduced into dikaryotic protoplasts of G.
View Article and Find Full Text PDFG3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration.
Cell Rep
February 2025
Yale Cardiovascular Research Center, Department of Internal Medicine, Section of Cardiology, Yale University School of Medicine, New Haven, CT 06511, USA; Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA. Electronic address:
The subcellular localization of mRNAs plays a pivotal role in biological processes, including cell migration. For instance, β-actin mRNA and its associated RNA-binding protein (RBP), ZBP1/IGF2BP1, are recruited to focal adhesions (FAs) to support localized β-actin synthesis, crucial for cell migration. However, whether other mRNAs and RBPs also localize at FAs remains unclear.
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