AI Article Synopsis
- Cell-penetrating peptides (CPPs) can transport biologically active molecules into cells, and a new CPP was identified from the C-terminus of human extracellular superoxide dismutase (hC-SOD3).
- Fluorescence microscopy revealed that this CPP localized in the cytoplasm and nucleus of HeLa cells, and when fused with apoptin, it effectively entered the cells and exhibited antitumor effects.
- Importantly, hC-SOD3 demonstrated the ability to deliver cargo without causing cytotoxicity at effective concentrations.
Article Abstract
Cell-penetrating peptides can carry a variety of biologically active molecules into cells. Here we have identified a novel CPP derived from the C-terminus of human extracellular superoxide dismutase (hC-SOD3) which was shown to be located throughout in the cytoplasm and nucleus by fluorescence microscopy investigation. Furthermore, when apoptin fused to hC-SOD3, it was translocated efficiently into HeLa cells resulting in antitumor activities. This study shows that hC-SOD3 has the potential to penetrate and translocate cargo molecules into cells and has no cytotoxicity at effective concentration.
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| http://dx.doi.org/10.1007/s11033-010-0406-6 | DOI Listing |
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