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Cytoskeletal mechanics of proplatelet maturation and platelet release. | LitMetric

AI Article Synopsis

  • Megakaryocytes create platelets by stretching their cytoplasm into proplatelet extensions, which act as factories for platelet production.
  • The study investigated how proplatelets mature and release into individual platelets, discovering various maturation stages and a new intermediate stage called preplatelet.
  • Preplatelets are small, round particles that can transform back into elongated proplatelets, with their release being influenced by the size of proplatelets and flowing conditions.

Article Abstract

Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, the terminal steps of proplatelet maturation and platelet release remain poorly understood. To elucidate this process, released proplatelets were isolated, and their conversion into individual platelets was assessed. This enabled us to (a) define and quantify the different stages in platelet maturation, (b) identify a new intermediate stage in platelet production, the preplatelet, (c) delineate the cytoskeletal mechanics involved in preplatelet/proplatelet interconversion, and (d) model proplatelet fission and platelet release. Preplatelets are anucleate discoid particles 2-10 µm across that have the capacity to convert reversibly into elongated proplatelets by twisting microtubule-based forces that can be visualized in proplatelets expressing GFP-β1-tubulin. The release of platelets from the ends of proplatelets occurs at an increasing rate in time during culture, as larger proplatelets undergo successive fission, and is potentiated by shear.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2983072PMC
http://dx.doi.org/10.1083/jcb.201006102DOI Listing

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