Interest is growing for a non-thermal sterilization technique in the food and pharmaceutical industries in order to ensure microbiological safety without the deterioration of product quality. In this study, supercritical nitrous oxide (SC N₂O) treatment was carried out in a multi-batch system to examine its bactericidal effect and characteristics, which largely remains unclear. The effect of operating pressure, temperature, mixing intensity, and working volume ratio (defined as the ratio of sample volume to the reactor volume to be filled with SC fluids) on the inactivation efficiency of SC N₂O were investigated in comparison with supercritical carbon dioxide (SC CO₂) treatment. Pseudomonas aeruginosa was chosen as a model microorganism. A 8-log reduction of P. aeruginosa cell concentration (10% working volume) in neutral phosphate-buffered saline was achieved by the SC N₂O treatment accompanying no pH change in the presence of vigorous mixing (600 rpm) within 6 min, in a condition of 37°C and 10 MPa and its overall efficiency is comparable to the SC CO₂ treatment. Among the reaction parameters investigated in this study, mixing intensity appeared to be the most important operating parameter affecting the bactericidal efficiency. The release of intracellular substances in cells as a result of the SC N₂O treatment was explained as one of major bactericidal actions by the SC N₂O treatment. At the same time, relatively negligible change of proteins or enzyme activities in the cells in case of the SC N₂O treatment was observed compared to the SC CO₂ treatment. Also, the observation by scanning and transmission electron microscopy shows the milder morphological change of SC N₂O-treated cells in comparison with the SC CO₂-treated cells. This study suggests that the SC N₂O bactericidal application on food products would be a viable option over SC CO₂ application, when mild change of enzyme activity and the proteins, or no pH change are desired.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijfoodmicro.2010.10.022 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Using Virtual Reality to Promote Self-Identity Reconstruction as the Main Focus of Therapy.
J Clin Psychol
January 2025
Department of Clinical Psychology and Psychobiology, The Institute of Neurosciences, Universitat de Barcelona, Barcelona, Spain.
Based on the repertory grid technique, we developed Explore Your Meanings (EYME), a digital platform that helps patients explore identity values and internal conflicts using virtual reality (VR). EYME was part of a research project treating depression in young adults, including 10 weekly, 1-h sessions aimed at changing personal constructs-cognitive schemas that shape how individuals interpret reality. We present the case of Mary, a 21-year-old woman diagnosed with persistent major depressive disorder and social phobia.
View Article and Find Full Text PDFEarly developmental trajectories of the impaired hand in infants with unilateral cerebral palsy.
Dev Med Child Neurol
January 2025
Queensland Cerebral Palsy and Rehabilitation Research Centre, Child Health Research Centre, Faculty of Medicine, The University of Queensland, Brisbane, Australia.
Aim: To identify developmental trajectories of impaired hand function in infants aged 3 to 15 months with unilateral cerebral palsy (CP).
Method: Sixty-three infants (37 male; median gestational age 37 weeks [interquartile range 30-39.1 weeks]) recruited as part of a randomized trial with a confirmed diagnosis of unilateral CP were included.
Intranasal administration of antiseizure drugs using new formulation trends: one step closer to reach clinical trials.
Expert Opin Drug Deliv
January 2025
CICS-UBI - Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal.
Introduction: Although there are numerous options for epilepsy treatment, its effective control continues unsatisfactory. Thus, search for alternative therapeutic options to improve the efficacy/safety binomial of drugs becomes very attractive to investigate. In this context, intranasal administration of antiseizure drugs formulated on state-of-the-art nanosystems can be a promising strategy.
View Article and Find Full Text PDFLow-Level Red Light for the Progression Myopia in Children: A Meta-Analysis.
Semin Ophthalmol
January 2025
Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, China.
Purposes: This meta-analysis aims to systematically analyze the efficacy of low-level red light (LRL) therapy for myopia control and prevention in children.
Methods: All the data were searched from the PubMed, EMBASE, and the Cochrane Library. The Cochrane Handbook was used to evaluate the quality of the included studies.
Circadian Misalignment Impacts Cardiac Autonomic Modulation in Adolescence.
Sleep
January 2025
Sleep Research & Treatment Center, Department of Psychiatry & Behavioral Health, Penn State University, College of Medicine, Hershey PA, USA.
Study Objectives: Although heart rate variability (HRV), a marker of cardiac autonomic modulation (CAM), is known to predict cardiovascular morbidity, the circadian timing of sleep (CTS) is also involved in autonomic modulation. We examined whether circadian misalignment is associated with blunted HRV in adolescents as a function of entrainment to school or on-breaks.
Methods: We evaluated 360 subjects from the Penn State Child Cohort (median 16y) who had at least 3-night at-home actigraphy (ACT), in-lab 9-h polysomnography (PSG) and 24-h Holter-monitoring heart rate variability (HRV) data.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!