AI Article Synopsis
- * A review of over 150 RNAi experiments reveals that RNAi is most effective in the Saturniidae family and immunity-related genes, while epidermal gene expression is more challenging to silence.
- * The study highlights the need for more research on RNAi mechanisms in Lepidoptera and its links to immune responses, with ongoing data collection to improve understanding through a public database.
Article Abstract
Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments.
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| http://dx.doi.org/10.1016/j.jinsphys.2010.11.006 | DOI Listing |
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