AI Article Synopsis
- Proviral sequences related to intracisternal A particles (IAP) are found throughout the mouse genome, showing a link between their expression and hypomethylation at specific CpG sites.
- Researchers utilized two-dimensional agarose gel electrophoresis to analyze the hypomethylation patterns of IAP in mouse DNA, focusing on specific sites important for detecting methylation status.
- Different inbred mouse strains displayed unique two-dimensional patterns of IAP-related restriction fragments, which were mostly consistent across tissues but showed some variations, indicating that these patterns are heritable and may serve as valuable markers for studying genomic methylation.
Article Abstract
Proviral sequences related to the intracisternal A particle (IAP) are amplified and dispersed in the mouse genome. Their expression is associated with hypomethylation at CpG sites in the 5' long terminal repeat. We have used two-dimensional agarose gel electrophoresis to examine patterns of IAP hypomethylation in mouse DNA. The method is sensitive to both the methylation status of a conserved Hae II site in the 5' long terminal repeat and the location of the closest BamHI site in the flanking DNA upstream of each hypomethylated long terminal repeat. The method also defects restriction fragments derived from IAP elements that are themselves methylated but have an unmethylated Hae II site in their 5' adjacent DNA. DNAs from each of four inbred mouse strains (BALB/c, C3H/He, C57BL/6, and DBA/2) gave distinctive two-dimensional patterns of BamHI/Hae II restriction fragments detected by hybridization with an IAP probe. This constitutive pattern was largely conserved among several tissues of each strain, but some tissue-specific variations were observed. The site-specific hypomethylations reflected in the two-dimensional patterns were heritable properties, since DNA from progeny of an interstrain cross contained both parental sets of fragments. IAP elements may be useful endogenous reporters of genomic methylation patterns.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC53668 | PMC |
| http://dx.doi.org/10.1073/pnas.87.6.2269 | DOI Listing |
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