The Duopath® Cereus Enterotoxins test (Merck KGaA) is a newly developed gold-labeled lateral flow immunoassay for the detection of Bacillus cereus enterotoxins. The test uses monoclonal antibodies (MAbs) against the L(2) component of hemolysin BL (Hbl) and NheB of the non-hemolytic enterotoxin (Nhe), respectively. The inclusivity and exclusivity of the assay was tested using 44 B. cereus, B. cereus group and Bacillus spp. strains. Apart from the B. mycoides type strain the results were in full agreement with those obtained by other immunological and molecular biological methods. The detection limit of the assay was 6ng/ml for NheB and 20ng/ml for the Hbl-L(2)-component, respectively. Using artificially and naturally contaminated food samples (n=76) the assay was positive after 18-24h enrichment if at least 10(2) enterotoxin producing B. cereus/g were present. After 30h enrichment samples contaminated with as low as 1 enterotoxin producing B. cereus/g gave positive results. In addition, testing of suspected colonies for enterotoxin production is possible. The assay is easy to perform and results can be clearly read without instrumentation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijfoodmicro.2010.10.008 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Potential Virulence and Survival of Acid-Resistant Bacillus cereus KS100Bc Isolated from Foodservice Establishments in Korea.
J Food Prot
January 2025
Food Safety Research Group, Korea Food Research Institute, Wanju 55365, Republic of Korea; Department of Food Biotechnology, University of Science and Technology, Daejeon 34113, Republic of Korea. Electronic address:
Bacillus cereus is an opportunistic pathogen causing food poisoning manifested by diarrheal or emetic syndromes. This study aimed to examine the potential virulence and survival of acid-resistant B. cereus strains isolated from foodservice establishments in schools.
View Article and Find Full Text PDFNatural Products Produced by the Species of Bacillus cereus Group: Recent Updates.
J Basic Microbiol
November 2024
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Puebla, Pue, Mexico.
Article Synopsis
- - The Bacillus cereus group produces a variety of antimicrobial compounds that help protect plants from pathogens and pests, while enhancing growth and stress tolerance.
- - These bacteria also play a crucial role in bioremediation, breaking down harmful environmental pollutants and producing beneficial enzymes, yet they can cause food poisoning due to enterotoxin production.
- - Overall, B. cereus has diverse applications in agriculture, industry, and medicine, including the development of eco-friendly materials and potential health benefits like cancer prevention through selenium nanoparticle production.
Identification and Validation of Garlic () Metabolites as Quorum Sensing Inhibitors of Targeting the PlcR Receptor: An and Study.
Foodborne Pathog Dis
October 2024
School of Chemical Engineering, Xiangtan University, Xiangtan, China.
This study aimed to investigate the influence of garlic metabolites on the quorum sensing (QS) of , a foodborne pathogen that controls its main virulence factor through QS. The QS signal receptor PlcR of was targeted by molecular docking with 82 garlic metabolites to identify the most potent QS inhibitors. Five metabolites, quercetin, kaempferol, luteolin, flavone, and rutin, were selected for further evaluation of their impacts on the growth, toxin production, and virulence of .
View Article and Find Full Text PDF.
Indian J Microbiol
September 2024
Department of Microbiology, Gauhati Medical College and Hospital, Guwahati, Assam 781032 India.
is an emerging food-borne pathogen responsible for two types of food poisoning: emetic and diarrhoeal type. Here we report an emetic type of food-borne illness attributable to On 2nd February, 2021, 202 people suffered from pain in abdomen and vomiting after consuming the rice provided during a public gathering in Diphu, Assam. Culture of leftover fried rice showed growth of group of organisms.
View Article and Find Full Text PDFA rapid and ultrasensitive paper sensor for Haemolysin BL detection.
Analyst
October 2024
State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
is a foodborne opportunistic pathogen commonly found in humans and animals. It produces various toxins, causing frequent food safety incidents. Therefore, establishing a fast and accurate method for detecting enterotoxin is crucial for disease diagnosis and food safety.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!