The range and extent of tear proteins removed by various multi-purpose solutions has been investigated, but there is little information in the literature about their ability to prevent denaturation of tear proteins, particularly lysozyme. The purpose of this study was to determine the ability of Bausch+Lomb Biotrue™ multi-purpose solution and other care solutions to affect denaturation of lysozyme using a lysozyme activity assay. The test solutions used were: Biotrue multi-purpose solution, Bausch+Lomb renu(®) fresh™, formerly ReNu MultiPlus(®), Alcon OPTI-FREE RepleniSH, Alcon OPTI-FREE EXPRESS, CIBA VISION AQuify, and AMO COMPLETE Multi-Purpose Solution Easy Rub Formula. A phosphate-buffered saline (PBS) solution served as a control. The test and control solutions containing lysozyme were exposed to sodium dodecyl sulfate (SDS), a known denaturant of the enzyme. The assay was based on digestion of the cell wall of Micrococcus luteus in a suspension, a substrate sensitive to active lysozyme. Enzymatic activity against M. luteus was used to assess activity of lysozyme. The decrease in the turbidity of the cell wall suspension, a measure of relative enzyme activity, was determined by following the decrease in absorbance (at 450nm) over time using a spectrophotometer. Statistically significant greater stabilization of lysozyme was observed with Biotrue multi-purpose solution and renu fresh than with OPTI-FREE RepleniSH, OPTI-FREE EXPRESS, AQuify, COMPLETE Multi-Purpose Solution Easy Rub Formula, and a PBS control. The lysozyme activity assay revealed that Biotrue multi-purpose solution and renu fresh have the ability to stabilize lysozyme under conditions that typically denature the protein.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.clae.2010.06.011 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Oxidized hyaluronic acid/ε-polylysine/quaternized chitosan hydrogel with shear thinning injectability and self-healing properties for full-time and multipurpose wound healing.
Int J Biol Macromol
January 2025
School of Pharmacy, Qingdao University, Qingdao 266071, China. Electronic address:
Complex wound closure scenarios necessitate the development of advanced wound dressings that can effectively address the challenges of filling irregularly shaped wounds and managing fatigue failures encountered in daily patient activities. To tackle these issues, we develop a multifunctional hydrogel from natural polysaccharides and polypeptides with injectability and self-healing properties for promoting full-time and multipurpose wound healing. Synthesized through dynamic Schiff base linkages between oxidized hyaluronic acid (OHA), ε-polylysine (ε-PL), and quaternized chitosan (QCS), the OHA/ε-PL/QCS hydrogel can gel rapidly within 50 s.
View Article and Find Full Text PDFDual-channel nano-carbon-liquid/liquid junction electrodes for multi-modal analysis: redox-active (dopamine) and non-redox-active (acetylcholine).
Analyst
December 2024
Department of Chemistry, The Beckman Institute for Advanced Science and Technology, University of Illinois Urbana Champaign, Urbana, IL 61801, USA.
We present here a dual-channel nanoelectrode to detect both redox-active and non-redox-active analytes. The dual-channel nanoelectrode was developed from theta nanopipette. We developed one channel of the theta nanopipette to be a carbon nanoelectrode and the other channel to be a nano interface between two immiscible electrolyte solutions (nanoITIES) electrode, producing a nano-carbon-ITIES platform.
View Article and Find Full Text PDFWhile traditional solutions for disposing of animal manure are limited by their time-consuming nature and inefficiency, the pyrolysis of animal manure into biochar is considered a promising disposal option, offering high-value benefits. However, there are few research studies on the physicochemical properties and potential utilization pathway of grub manure-derived biochar (GB) prepared at different temperatures. In this study, grub manure (GM) was pyrolyzed at 450, 600 and 750 °C, and the effect of pyrolysis temperature on the characteristics and applications of GB was illustrated.
View Article and Find Full Text PDFFirst Report of Causing Leaf Anthracnose on L in China.
Plant Dis
August 2024
Guangdong Ocean University, College of Coastal Agriculture Sciences, Department of Biotechnology，Agricultural College，Guangdong Ocean University，Huguang Yan, Zhanjiang, China, 524088;
Article Synopsis
- Jatropha curcas L., a versatile crop used for disease treatment and environmental restoration, is gaining attention for its oil, which can be used to produce biodiesel.
- In June 2023, a leaf disease affecting around 60% of J. curcas was reported in Mazhang, Guangdong Province, displaying anthracnose symptoms.
- The disease was studied by culturing infected tissue on potato dextrose agar, leading to the identification of Colletotrichum sp. through morphological characteristics and genetic sequencing.
"Killing two birds with one stone" - a qualitative study on women's perspectives on the dual prevention pill in Johannesburg, South Africa.
BMC Womens Health
August 2024
Social and Behavioral Research, Population Council, Washington, DC, USA.
Background: HIV incidence remains high in South Africa, with ~ 60% of all new HIV infections among adolescent girls and women (Country factsheets HIV and AIDS Estimates, 2022). Oral pre-exposure prophylaxis (PrEP), approved for HIV prevention in South Africa since 2015, is hampered by low uptake and adherence, particularly among adolescent girls and young women (AGYW). Combining oral PrEP with oral contraceptives could increase PrEP uptake, persistence and address unmet needs for contraception.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!