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Unlabelled: Thiosulfate-citrate-bile salts-sucrose (TCBS) agar is a selective and differential media for the enrichment of pathogenic . We observed that an exonuclease VII ( ) mutant of failed to grow on TCBS agar, suggesting that DNA repair mutant strains may be hampered for growth in this selective media. Examination of the selective components of TCBS revealed that bile acids were primarily responsible for toxicity of the mutant.
View Article and Find Full Text PDFChemically reduced graphene oxide based assembly of Aptasensor for sensitive and probe-free detection of penicillin-G.
Food Chem
January 2025
BRIC-National Institute of Animal Biotechnology (NIAB), Hyderabad 500032, Telangana, India; BRIC- Regional Centre for Biotechnology (RCB), Faridabad 121001, Haryana, India.. Electronic address:
The widespread use of antibiotics in livestock and poultry leads to antibiotic residues in food, posing public health risks. To ensure food safety, monitoring antibiotic levels in dairy and poultry is essential, especially for Penicillin-G (Pen-G), a frequently used β-lactam antibiotic. This study presents an electrochemical aptasensor for detecting Pen-G in food samples, using chemically reduced graphene oxide (crGO) conjugated with Pen-G-specific aptamer on electrode.
View Article and Find Full Text PDFTHSWD upregulates the LTF/AMPK/mTOR/Becn1 axis and promotes lysosomal autophagy in hepatocellular carcinoma cells by regulating gut flora and metabolic reprogramming.
Int Immunopharmacol
January 2025
Department of Hepatology, Southern Medical University Hospital of Integrated Traditional Chinese and Western Medicine, Southern Medical University, 510315 Guangzhou, China. Electronic address:
THSWD has the effect of reducing inflammation, improving microcirculation, and regulating immune status in patients with hepatocellular carcinoma. Regardless of its clear therapeutic effect, the underlying mechanism of action against hepatocellular carcinoma is not clear. To identify critical gut microbiota and its associated metabolites related to THSWD inhibition against hepatocellular carcinoma progression, we assessed the microbe-dependent anti-hepatocellular carcinoma effects of THSWD through 16 s rRNA gene sequencing, fecal microbial transplantation and antibiotic treatment.
View Article and Find Full Text PDFCystitis glandularis due to Escherichia coli infection in a diabetic Miniature Schnauzer: a case report.
BMC Vet Res
January 2025
Faculty of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina.
Background: Lower urinary tract disease is a common clinical condition in dogs, usually presenting with dysuria, pollakiuria and haematuria. Diabetes mellitus is a predisposing factor for urinary tract infection in both humans and dogs and does not necessarily present with clinical signs. In this case report, we describe for the first time a case of cystitis glandularis in a dog with diabetes mellitus, associated with Escherichia coli urinary tract infection.
View Article and Find Full Text PDFPeriorificial dermatitis in infants and preschoolers - a narrative review.
Eur J Pediatr
January 2025
Department of Dermatology & Allergology, Städtisches Klinikum Dresden, Academic Teaching Hospital, Dresden, Germany.
Periorifical dermatitis (POD) is a papular, chronic inflammatory skin disease commonly seen in women in their 2nd to 4th decade of life. The major differential diagnosis is persistent acne. In children, POD is less common than in adults.
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