Metabolism mediated interaction of α-asarone and Acorus calamus with CYP3A4 and CYP2D6.

Fitoterapia

School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.

Published: April 2011

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Article Abstract

The present study was aimed to investigate the possible interaction of the standardized extract of Acorus calamus (AC) with Cytochrome P450 enzyme, quantitative determination of the α-asarone in the AC rhizome was performed by RP-HPLC method. In vitro interaction of the plant extract was evaluated by CYP450-carbon monoxide complex (CYP450-CO) assay. Effect on individual isoforms such as CYP3A4 and CYP2D6 isozymes were analyzed through fluorescence product formation and respective IC(50) values were determined. CYP450-CO assay showed moderate interaction potential. Extract showed higher IC(50) values (46.84±1.83-32.99±2.21 μg/ml) comparing to the standard inhibitors and lower IC(50) value than α-asarone (65.16±2.37-42.15±2.45 μg/ml).

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http://dx.doi.org/10.1016/j.fitote.2010.11.009DOI Listing

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