AI Article Synopsis
- The study aimed to assess how different wound types and locations affect the production of COMP and TGF-β1 during the healing process in horses.
- Six Standardbred horses were used to create identical full-thickness skin wounds, with samples taken at various healing stages to measure COMP and TGF-β1 levels.
- Results showed that while COMP levels were higher in intact neck skin compared to the metacarpus, neither the type or site of the wounds influenced COMP or TGF-β1 production, and no correlation was found between the two proteins during healing.
Article Abstract
Objective: To evaluate whether wound type or site influence the production of cartilage oligomeric matrix protein (COMP) and transforming growth factor β1 (TGF-β1), and determine if there is a correlation between TGF-β1and COMP during healing.
Study Design: Experimental.
Animals: Standardbred horses (n=6), 4-8 years old.
Methods: Six, standardized, full-thickness skin wounds (open, straight, and elliptical) were surgically created on the neck (n=3) and metacarpus (3) on each horse. Wounds were randomly allocated to site and side. Tissue samples were collected before creating wounds and on days 7, 14, and 42. COMP concentration (μg/g dry weight of tissue) was determined using a standard competitive ELISA and TGF-β1 (ng/g dry weight of tissue) was determined using a commercially available sandwich ELISA.
Results: COMP concentrations were higher in intact skin on the neck compared with the metacarpus (P=.02). There was no difference in COMP and TGF-β1 concentrations between the different wound types or sites during healing. There was no correlation between TGF-β1 and COMP during healing.
Conclusions: Within the limitations of the study design, production of COMP during healing of skin wounds does not appear to be influenced by wound type or anatomic site, nor does it appear to be correlated with TGF-β1 concentrations.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1532-950X.2010.00756.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification of 8-(2-methyl phenyl)-9H-benzo[f]indeno[2,1-c]quinolin-9-one (C-5635020) as a novel and selective TGFβ RII kinase inhibitor for breast cancer therapy.
Biochem Biophys Res Commun
January 2025
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia. Electronic address:
Objective And Significance: Transforming growth factor-beta (TGF-β) plays a pivotal role in breast development by modulating tissue composition during the developmental phase. The TGFβ type II receptor (TGFβ RII) is implicated in breast cancer and represents a valuable therapeutic target. Due to the off-target side effects of many existing TGFβI/TGFβ RII inhibitors, a more targeted approach to drug discovery is necessary.
View Article and Find Full Text PDFFeatures of Metabolism and Its Regulation in the Dynamics of Experimental Models of Metabolic Disorders.
Bull Exp Biol Med
January 2025
Center for Digital and Translational Biomedicine, Center for Molecular Health LLC, Moscow, Russia.
Changes in the lipid and carbohydrate metabolism, adipokines, and growth factors during the development of metabolic disorders were studied in three mouse models: C57BL/6 (alimentary obesity), db/db (leptin-resistant obesity), and NOD (diabetes mellitus) lines. In the group of alimentary obesity, moderate fatty infiltration of the liver and hypertrophy of the adipose tissue, hyperglycemia, and increased concentrations of adiponectin, transforming growth factor β1 (TGF-β1), leptin, and cholesterol were detected. In the group of leptin-resistant obesity, multiple pathological changes in tissues, severe hyperglycemia and hyperleptinemia, hyperinsulinemia, and reduced concentrations of triglycerides, adiponectin, myostatin, and TGF-β1 were detected.
View Article and Find Full Text PDFCircadian clock gene BMAL1 is involved in transforming growth factor β1-induced fibrotic response in NRK-49F cells.
Cell Biol Int
January 2025
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokkaido University of Science, Teine-ku, Japan.
The transcription factor brain and muscle Arnt-like protein-1 (BMAL1) is a clock protein involved in various diseases, including atherosclerosis and cancer. However, BMAL1's involvement in kidney fibrosis and the underlying mechanisms remain largely unknown, a gap addressed in this study. Analysis through Masson's trichrome and Sirius red staining revealed that all groups exposed to unilateral ureteral obstruction showed increased BMAL1 protein expression accompanied by increased TGF-β1 expression and elevated key fibrosis markers, including α-SMA, compared with sham groups.
View Article and Find Full Text PDFReduced lung metastasis in endothelial cell-specific transforming growth factor β type II receptor-deficient mice with decreased CD44 expression.
iScience
December 2024
Laboratory of Stem Cell Regulation, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Transforming growth factor β (TGF-β) is abundantly present in the tumor microenvironment, contributing to cancer progression. However, the regulatory mechanism by which TGF-β affects vascular endothelial cells (ECs) in the tumor microenvironment is not well understood. Herein, we generated tamoxifen-inducible TGF-β type II receptor () knockout mice, specifically targeting ECs (TβRII), by crossbreeding TβRII-floxed mice with Pdgfb-icreER mice.
View Article and Find Full Text PDFThe hepatoprotective effects of the polyphenol-enriched n-butanol fraction of against carbon tetrachloride-induced liver fibrosis in rats: study.
Toxicol Rep
June 2025
Department of Pharmacology and Toxicology, College of Pharmacy, Al-Nahrain University, Baghdad, Iraq.
Liver fibrosis is a continuous wound-healing response to chronic injury caused by various chemical, virus, and pathological disorders; the lack of approved drugs or methods to reverse or prevent liver fibrosis makes it an interesting area of research. This study investigates the potential hepatoprotective effects of the phenolic extract of in rat's module of liver fibrosis. Liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride (CCl) for six consecutive weeks; the butanol fraction of and silymarin was administered orally concurrently with CCl.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!