The nuclear matrix-intermediate filament system of human neuroblastoma SK-N-SH cells before and after retinoic acid (RA) treatment was selectively extracted and the distribution of prohibitin (PHB) in the nuclear matrix, as well as its colocalization with related genes, was observed. Results of two-dimensional gel electrophoresis (2-DE), mass spectrometry (MS) identification, and protein immunoblotting all confirm that PHB was present in the components of SK-N-SH nuclear matrix proteins and was down-regulated after RA treatment. Immunofluorescence microscopy observations show that PHB was localized in the nuclear matrix and its distribution was altered due to RA treatment. Laser confocal microscopy results reveal that PHB colocalized with the expression products of c-myc, c-fos, p53, and Rb, but the colocalization region was altered after RA treatment. Our results prove that PHB is a nuclear matrix protein and is localized in nuclear matrix fibers. The distribution of PHB in SK-N-SH cells and its colocalization with related proto-oncogenes and tumor suppressor genes suggest that PHB plays pivotal roles in the differentiation of SK-N-SH cells and deserves further study.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10571-010-9608-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chronically persistent viruses are integral components of the organismal ecosystem in humans and animals . Many of these viruses replicate and accumulate within the cell nucleus . The nuclear location allows viruses to evade cytoplasmic host viral sensors and promotes viral replication .
View Article and Find Full Text PDFPhysical Exercise: A Promising Treatment Against Organ Fibrosis.
Int J Mol Sci
January 2025
College of Physical Education, Shanghai University, Shanghai 200444, China.
Fibrosis represents a terminal pathological manifestation encountered in numerous chronic diseases. The process involves the persistent infiltration of inflammatory cells, the transdifferentiation of fibroblasts into myofibroblasts, and the excessive deposition of extracellular matrix (ECM) within damaged tissues, all of which are characteristic features of organ fibrosis. Extensive documentation exists on fibrosis occurrence in vital organs such as the liver, heart, lungs, kidneys, and skeletal muscles, elucidating its underlying pathological mechanisms.
View Article and Find Full Text PDFImproving Corrosion Resistance of Zircaloy-4 via High-Current Pulsed Electron Beam Surface Irradiation.
Materials (Basel)
December 2024
Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, College of Mechanical and Electrical Engineering, Changchun University of Science and Technology, Changchun 130022, China.
Zircaloy-4 is extensively used in nuclear reactors as fuel element cladding and core structural material. However, the safety concerns post-Fukushima underscore the need for further enhancing its high-temperature and high-pressure water-side corrosion resistance. Therefore, this study aimed to investigate the effects of high-current pulsed electron beam (HCPEB) irradiation on the microstructures and corrosion resistance of Zircaloy-4, with the goal of improving its performance in nuclear applications.
View Article and Find Full Text PDFClick hydrogels to assess stiffness-induced activation of pancreatic cancer-associated fibroblasts and its impact on cancer cell spreading.
Chembiochem
January 2025
Purdue University College of Engineering, Weldon School of Biomedical Engineering, 723 W. Michigan St., SL 220K, IN 46202, Indianapolis, UNITED STATES OF AMERICA.
Pancreatic ductal adenocarcinoma (PDAC) is marked by significant desmoplastic reactions, or the accumulation of excessive extracellular matrices. PDAC stroma has abnormally high stiffness, which alters cancer cell behaviors and creates a barrier for effective drug delivery. Unfortunately, clinical trials using a combination of chemotherapy and matrix-degrading enzyme have led to disappointing results, as the degradation of stromal tissue likely accelerated the dissemination of cancer cells.
View Article and Find Full Text PDFSATB2 Expression Affects Chemotherapy Metabolism and Immune Checkpoint Gene Expression in Colorectal Cancer.
Clin Colorectal Cancer
December 2024
Department of Colorectal Surgery, Beaumont Hospital, Dublin, Ireland. Electronic address:
Background: Special AT-rich binding protein-2 (SATB2) is a nuclear matrix associated protein regulating gene expression which is normally expressed in colonic tissue. Loss of SATB2 expression in colorectal cancer (CRC) has negative implications for prognosis and has been associated with chemotherapy resistance. Furthermore, recent evidence suggests SATB2 may influence immune checkpoint (IC) expression.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!