This unit describes a general protocol for the isolation of Streptomyces species from soil and fresh water, using a procedure for the selective growth of Streptomyces species. Preparation of the necessary growth medium, recognition of the morphology of the bacteria, and safety considerations are also covered.
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Interactions between common scab-inducing strains on potato suberin.
Can J Microbiol
March 2025
Université de Sherbrooke, Département de Biologie, Sherbrooke, Quebec, Canada;
Potato tuber periderm is armored with suberin, that consists of two domains, an aliphatic domain composed of fatty acid polyesters and an aromatic domain composed of cinnamic acids. Streptomyces scabies 87.22, a predominant causal agent of potato common scab, was compared for adaptation to tuber suberin with Streptomyces acidiscabies ATCC 49003 and Streptomyces turgidiscabies Car8 belonging to emerging pathogenic species.
View Article and Find Full Text PDFPolyphasic taxonomic description of sp. nov. and sp. nov. and their biotechnological potential.
Int J Syst Evol Microbiol
March 2025
German Collection of Microorganisms and Cell Cultures, Leibniz Institute DSMZ, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
strains DSM 116494 and DSM 116496 were isolated from sediment samples of the River Oker in Braunschweig, Germany, and subjected to a polyphasic taxonomic study and genome mining for specialized secondary metabolites. Phenotypic, genetic and genomic data confirmed the assignment of these strains to the genus. Pairwise 16S rRNA gene sequence similarity values between the strains and validly named species reached 99.
View Article and Find Full Text PDFEffects of Geosmin on the Behavior of Soil Protists.
Microb Ecol
March 2025
Cooperative Institute for Research in Environmental Sciences, University of Colorado Boulder, Boulder, CO, USA.
Geosmin is a volatile organic compound (VOC) produced by a range of different soil microorganisms, and is most commonly recognized for its characteristic "earthy" scent evident after rainfall. Though it remains unclear why microorganisms produce geosmin, we know that exposure to geosmin can influence behaviors across a wide range of organisms, serving as both an attractant and a repellant, but geosmin effects on soil protists remain largely unstudied. We investigated how soil protists respond to geosmin exposures, focusing on representatives of three morphological groups of protists, Colpoda sp.
View Article and Find Full Text PDFComplete Genome Sequence, Biological Activities, and Metabolomic Profiles of Mangrove-Derived Streptomyces sp. SM1P.
Curr Microbiol
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Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR, 97331, USA.
The genome of Streptomyces sp. SM1P was sequenced to give a contig of 7,561,428 bp (71.7% G + C content) and 24 putative secondary metabolite biosynthetic gene clusters (BGCs).
View Article and Find Full Text PDFAntibacterial and cytotoxic angucyclines discovered by heterologous expression of a type II polyketide gene cluster.
Org Biomol Chem
March 2025
Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, People's Republic of China.
Three new aromatic polyketides, spirocyclione C (1) and angumycinones E and F (2 and3), were isolated from the heterologous expression of a type II gene cluster in the strain of A3(2) assisted by the one strain many compounds (OSMAC) strategy. The structures and absolute configurations of these compounds were elucidated by extensive NMR, MS, theoretical NMR calculations, DP4+ probability analysis, and ECD analyses. Notably, compound 1 represents the first example of an angucycline structure with an unusual oxaspiro[5.
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