The significance of mastocytaemia in cats is different from that in dogs because it appears exclusively associated with mast cell neoplasia. The prevalence of mastocytaemia was 0.05% of all feline submissions to a private laboratory and 43% in cats with mast cell neoplasia. None of 30 healthy cats had mastocytaemia. There was no sex bias or significant age difference between mastocytaemic and non-mastocytaemic cats with mast cell tumours (MCT). Buffy coat (BC) examination was the best screening method for detection of mastocytaemia but direct blood film examination was more accurate for quantifying degree of mastocytaemia. BC examination should be performed in all cases of suspected/known mast cell neoplasia as mastocytaemia was missed on nearly 30% of occasions when direct film examination only was used. Mastocytaemia was associated with decreased haematocrit (HCT) but not with other haematological parameters. Mastocytaemic cats can survive significant lengths of time (up to 27 months) even when splenectomy is not performed.
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http://dx.doi.org/10.1016/j.jfms.2010.08.003 | DOI Listing |
Front Immunol
December 2024
KU Leuven Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Group, Leuven, Belgium.
Primary human mast cells (MC) obtained through culturing of blood-derived MC progenitors are the preferred model for the study of MRGPRX2- IgE-mediated MC activation. In order to assess the impact of culture conditions on functional MRGPRX2 expression, we cultured CD34-enriched PBMC from peripheral whole blood (PB) and buffy coat (BC) samples in MethoCult medium containing stem cell factor (SCF) and interleukin (IL)-3, modified through variations in seeding density and adding or withholding IL-6, IL-9 and fetal bovine serum (FBS). Functional expression of MRGPRX2 was assessed after 4 weeks via flow cytometry.
View Article and Find Full Text PDFOpen Vet J
November 2024
Division of Animal Medical Research, Hassen-kai, 2-27 Onozaki, Saito, Miyazaki 881-0012, Japan.
Background: Canine mast cell tumors (MCT) in the skin are classified into cutaneous MCT (cMCT) and subcutaneous MCT (scMCT) types, which exhibit different clinical behaviors. Although these types have been classified only by histology, preoperative differentiation is important for proper surgical planning.
Aim: To examine the accuracy of differentiating these types based on the gross features before surgery.
Int Immunopharmacol
December 2024
Department of Dermatology, The First Hospital of China Medical University, Shenyang, China; NHC Key Laboratory of Immunodermatology, Ministry of Education Key Laboratory of Immunodermatology, National Joint Engineering Research Center for Diagnosis and Treatment of Immunologic Skin Diseases, The First Hospital of China Medical University, Shenyang, China.
Background: Activation of the aryl hydrocarbon receptor (AhR) ameliorates LL-37-induced rosacea-like dermatitis in mice, whereas mast cells and cytokine overexpression are prominent features in rosacea skin.
Objective: To evaluate the potential mechanisms of AhR activation on autophagy and degranulation of mast cells in rosacea.
Methods: LL-37 treated mast cells were used to mimic rosacea.
Front Immunol
December 2024
School of Optometry, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, China.
Background: Thyroid-associated orbitopathy (TAO) is an autoimmune inflammatory disorder of the orbital adipose tissue, primarily causing oxidative stress injury and tissue remodeling in the orbital connective tissue. Ferroptosis is a form of programmed cell death driven by the accumulation of reactive oxygen species (ROS), iron metabolism disorder, and lipid peroxidation. This study aims to identify and validate the optimal feature genes (OFGs) of ferroptosis with diagnostic and therapeutic potential in TAO orbital adipose tissue through bioinformatics analysis and to assess their correlation with disease-related immune cell infiltration.
View Article and Find Full Text PDFTurk J Med Sci
December 2024
Department of Pathology, Faculty of Veterinary Medicine, Bursa Uludağ University, Bursa, Turkiye.
Background/aim: Breast cancers are one of the most common cancers in women and are responsible for many deaths worldwide. Mast cells are inflammatory cells. Their role in cancers is controversial, and there is limited data on systemic mast cell activation in cancer cases.
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