AI Article Synopsis
Article Abstract
The distribution of some proteoglycan anionic sites and carbohydrate moieties on the diabetic platelet surface has been investigated by cytochemical methods. Human blood platelets obtained from either diabetic patients (serum glycaemia 9.6-19.2 mmol/L) or normal donors were labeled with specific stains (Ruthenium Red, Safranin O) or with biotinylated lectins (WGA, RCA(120))/Straptavidin-gold. All the probes were processed in a standardised manner for transmission electron microscopy. Results showed a modified pattern of diabetic platelet surface labeling as compared to normals. The anionic sites in the glycocalyx (particularly proteoglycans), evenly distributed all over the normal platelet plasma membrane, suffered a marked redistribution, with cluster formation, on the surface of diabetic platelets. The sugar moieties were also differently exposed: as compared to normal, diabetic platelets expressed a 1.5-fold lower number of WGA-binding sites (sialic acid) and a 2.5-fold higher number of RCA(120)-binding sites (galactose residues). We conclude that the distribution of some platelet surface components, potentially implicated in the adhesion and aggregation processes, is significantly modified on platelets from diabetic patients.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3109/09537109409006047 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Atomistic Mechanism of Lipid Membrane Binding for Blood Coagulation Factor VIII with Molecular Dynamics Simulations on a Microsecond Time Scale.
J Phys Chem B
January 2025
Chemistry Department, Western Washington University, Bellingham, Washington 98225-9038, United States.
During the blood coagulation cascade, coagulation factor VIII (FVIII) is activated by thrombin to form activated factor VIII (FVIIIa). FVIIIa associates with platelet surfaces at the site of vascular damage to form an intrinsic tenase complex with activated factor IX. A working model for FVIII membrane binding involves the association of positively charged FVIII residues with negatively charged lipid headgroups and the burial of hydrophobic residues into the membrane interior.
View Article and Find Full Text PDFF-FAPI-42 PET/CT for preoperatively identifying intrahepatic cholangiocarcinoma and hepatocellular carcinoma.
Quant Imaging Med Surg
January 2025
Department of Nuclear Medicine, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Background: Accurately differentiating hepatocellular carcinoma (HCC) from intrahepatic cholangiocarcinoma (ICC) is essential for therapeutic decision-making. This study aimed to explore the value of Fluor 18 (F)-conjugated fibroblast-activation protein inhibitor (FAPI-42) positron emission tomography-computed tomography (PET/CT) in distinguishing HCC from ICC preoperatively.
Methods: Patients with suspected intrahepatic lesions who underwent F-FAPI-42 PET/CT were retrospectively assessed and placed into an HCC group and an ICC group based on postoperative pathology.
Biophysiochemically favorable, antithrombotic and pro-endothelial coordination compound nanocoating of copper (II) with protocatechuic acid & nattokinase on flow-diverting stents.
Colloids Surf B Biointerfaces
January 2025
Institute of Biomedical Engineering, College of Medicine, Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China. Electronic address:
Neurovascular flow-diverting stents (FDSs) are revolutionizing the paradigm for treatment of intracranial aneurysms, but they still face great challenges like post- implantation acute thrombosis and delayed reendothelialization. Surface modification is of crucial relevance in addressing such key issues. In this study, we fabricated an ultrathin nanocoating out of copper (II) together with protocatechuic acid (PCA) and nattokinase (NK) bioactive molecules on NiTi FDSs via a coordination chemistry approach, with favorable biophysiochemical interactions, to fulfill this goal.
View Article and Find Full Text PDFAutonomous Anticoagulation on a Biomimetic Al-Based Hierarchical Surface.
ACS Appl Mater Interfaces
January 2025
Key Laboratory of Bionic Engineering Ministry of Education, Jilin University, Changchun 130025, China.
Studies targeting the blood repellency and autonomous anticoagulation of superhydrophobic (SH) surfaces are potentially valuable for their application in blood contact. The anticoagulation abilities and potential mechanisms of different SH surfaces urgently need to be revealed. In this study, a range of microprotrusion arrays on Al substrates with varying spacings via laser ablation through the utilization of organic adsorption and siloxane coupling reactions were fabricated.
View Article and Find Full Text PDFImpact of Platelet Transfusion at Different Doses in Oncohematology Pediatric Inpatients and Outpatients: A Retrospective Study.
Pediatr Blood Cancer
January 2025
Transfusion Medicine and Cellular Therapy Unit, Policlinico Campus Bio-Medico Foundation, Rome, Italy.
Background: Platelet (PLT) transfusion is an essential strategy to prevent bleeding in children with thrombocytopenia associated to cancer treatment. However, data on optimal pediatric dosing and transfusion thresholds are limited.
Methods: This retrospective study analyzed data from 607 pediatric patients with hematologic malignancies, nonmalignant disorders, and solid tumors who developed hypoproliferative thrombocytopenia during therapy.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!