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PathoGD: an integrative genomics approach to primer and guide RNA design for CRISPR-based diagnostics.
Commun Biol
January 2025
Department of Infectious Diseases, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.
Critical to the success of CRISPR-based diagnostic assays is the selection of a diagnostic target highly specific to the organism of interest, a process often requiring iterative cycles of manual selection, optimisation, and redesign. Here we present PathoGD, a bioinformatic pipeline for rapid and high-throughput design of RPA primers and gRNAs for CRISPR-Cas12a-based pathogen detection. PathoGD is fully automated, leverages publicly available sequences and is scalable to large datasets, allowing rapid continuous monitoring and validation of primer/gRNA sets to ensure ongoing assay relevance.
View Article and Find Full Text PDFMitigating cadmium stress in rice (Oryza sativa L.) using succinic and oxalic acids with focus on cellular integrity and antioxidant responses.
Plant Physiol Biochem
January 2025
Department of Environmental Sciences, Government College University Allama Iqbal Road, Faisalabad 38000, Pakistan; Department of Biological Sciences and Technology, China Medical University, Taichung, 40402, Taiwan. Electronic address:
Soil contamination with toxic heavy metals [such as cadmium (Cd)] is becoming a serious global problem due to the rapid development of the social economy. Organic chelating agents such as succinic acid (SA) and oxalic acid (OA) are more efficient, environmentally friendly, and biodegradable compared to inorganic chelating agents and they enhance the solubility, absorption, and stability of metals. To investigate this, we conducted a pot experiment to assess the impact of SA (0.
View Article and Find Full Text PDFFlexible multifunctional Janus film towards self-healing, self-adhesive and streachable EMI shielding.
J Colloid Interface Sci
January 2025
Shaanxi Key Laboratory of Chemical Additives for Industry, College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China. Electronic address:
As smart electronic devices proliferate rapidly, concerns about electromagnetic radiation have become more prominent. Traditional electromagnetic shielding materials typically use three-dimensional porous foams, carbon structures, and film materials as their substrates. However, as electronic devices become more miniaturized, integrated, and precise, the large volume and limited functionality of foam materials have constrained their applications.
View Article and Find Full Text PDFIntegration of Rapid Cycle Deliberate Practice Into Prelicensure Nursing Curricula: Preparing Nursing Students for Cardiac Arrest Care.
Nurse Educ
January 2025
Authors Affiliations: Department of Biobehavioral Nursing Science (Drs Castro, Stephens, and Vanderzwan), Department of Human Development Nursing Science (Ms Ortiz), College of Nursing, University of Illinois at Chicago, Chicago, IL.
Background: Nursing students are not consistently trained on how to care for patients experiencing sudden cardiac arrest. In rapid cycle deliberate practice (RCDP), learners perform a simulation with micro-debriefs interjected by the instructor.
Problem: RCDP has been used to train health care students on how to respond during a sudden cardiac arrest, but its application to prelicensure nursing students is underreported.
Rapid Electrochemical Uranium Extraction from Real Seawater via the Intermediate of Vacancy-Trapped Isolated Uranyl.
Inorg Chem
January 2025
State Key Laboratory of Environment-friendly Energy Materials, School of National Defence Science & Technology, Nuclear Waste and Environmental Safety Key Laboratory of Defense, Southwest University of Science and Technology, Mianyang, Sichuan 621010, P. R. China.
Electrochemical uranium extraction from seawater is a vital project for the sustainable development of the nuclear industry, which requires selective intrinsic binding sites for uranyl. In this work, oxygen vacancies (O vacancies) were developed as an atomically identified confinement for uranyl, and thus, rapid uranium extraction from seawater was achieved. In a short period of 700 s, InO nanosheets with rich O vacancies (V-rich InO nanosheets) exhibited a high extraction efficiency of 88.
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