AI Article Synopsis
- The study focused on the variety of nitrogen-fixing bacteria and their activity in Italian white truffles (Tuber magnatum).
- Researchers used specific PCR primers to detect the nitrogenase gene nifH at various truffle maturation stages, finding that most bacteria belonged to the Bradyrhizobium group.
- The measured nitrogenase activity indicated levels comparable to early legume nodules, marking the first evidence of nitrogenase gene expression and activity in truffles.
Article Abstract
Diversity of nitrogen-fixing bacteria and the nitrogen-fixation activity was investigated in Tuber magnatum, the most well-known prized species of Italian white truffle. Degenerate PCR primers were applied to amplify the nitrogenase gene nifH from T. magnatum ascomata at different stages of maturation. Putative amino acid sequences revealed mainly the presence of Alphaproteobacteria belonging to Bradyrhizobium spp. and expression of nifH genes from Bradyrhizobia was detected. The nitrogenase activity evaluated by acetylene reduction assay was 0.5-7.5μmolC(2)H(4)h(-1)g(-1), comparable with early nodules of legumes associated with specific nitrogen-fixing bacteria. This is the first demonstration of nitrogenase expression gene and activity within truffle.
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| http://dx.doi.org/10.1016/j.funbio.2010.09.001 | DOI Listing |
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