Two complementary methods were optimized for the separation and detection of trace levels of hydrogen peroxide. The first method utilized reversed-phase high-performance liquid chromatography with fluorescence detection (HPLC-FD). With this approach, hydrogen peroxide was detected based upon its participation in the hemin-catalyzed oxidation of p-hydroxyphenylacetic acid to yield the fluorescent dimer. The second method utilized high performance liquid chromatography with electrochemical detection (HPLC-ED). With this approach, hydrogen peroxide was detected based upon its oxidation at a gold working electrode at an applied potential of 400 mV vs. hydrogen reference electrode (Pd/H(2)). Both methods were linear across the range of 15-300 μM, and the electrochemical method was linear across a wider range of 7.4-15,000 μM. The limit of detection for hydrogen peroxide was 6 μM by HPLC/FD, and 0.6 μM by HPLC/ED. A series of organic peroxides and inorganic ions were evaluated for their potential to interfere with the detection of hydrogen peroxide. Studies investigating the recovery of hydrogen peroxide with three different extraction protocols were also performed. Post-blast debris from the detonation of a mixture of concentrated hydrogen peroxide with nitromethane was analyzed on both systems. Hydrogen peroxide residues were successfully detected on this post-blast debris.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.chroma.2010.10.022 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Vacancy-Activated B-Doping for Efficient 2e- Oxygen Reduction through Suppressing H2O2 Decomposition at High Overpotential.
Angew Chem Int Ed Engl
January 2025
China University of Petroleum East China, State Key Laboratory of Heavy Oil Processing, 66 The Yangtze River West Road, 266580, Qingdao, CHINA.
The production of hydrogen peroxide (H2O2) through two-electron oxygen reduction reaction (2e- ORR) has emerged as a more environmentally friendly alternative to the traditional anthraquinone method. Although oxidized carbon catalysts have intensive developed due to their high selectivity and activity, the yield and conversion rate of H2O2 under high overpotential still limited. The produced H2O2 was rapidly consumed by the increased intensity of H2O2 reduction, which could ascribe to decomposition of peroxide radicals under high voltage in the carbon catalyst.
View Article and Find Full Text PDFCationizable transcytosis manganese nano-oxygenator for enhanced chemo-dynamic immunotherapy in deep tumour tissue.
J Mater Chem B
January 2025
Department of Oncology, Yunfu People's Hospital, Yunfu 527300, Guang Dong, China.
Effective delivery of therapeutic agents for solid tumour treatment is impeded by multiple obstacles, such as aberrant interstitial fluid pressure and high density of the extracellular matrix, which causes impaired penetration to deep avascular tumour tissue that exists in a hypoxic immune cold environment. Only limited tumoricidal effects have been achieved with traditional nanomedicine due to its inefficient penetration and the multiple resistant effects that exist in the tumour microenvironment. Herein, a new chemo-dynamic immunotherapy (CDIT) is proposed based on a transcytosis tumour oxygenator (MnP) with effective chemo-dynamic effects.
View Article and Find Full Text PDFChronic Jet Lag Disrupts Circadian Rhythms and Induces Hyperproliferation in Murine Lacrimal Glands via ROS Accumulation.
Invest Ophthalmol Vis Sci
January 2025
Department of Ophthalmology, People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, Zhengzhou, China.
Purpose: Chronic jet lag (CJL) is known to disrupt circadian rhythms, which regulate various physiological processes, including ocular surface homeostasis. However, the specific effects of CJL on lacrimal gland function and the underlying cellular mechanisms remain poorly understood.
Methods: A CJL model was established using C57BL/6J mice.
Co-encapsulation of Creatininase, Creatinase, and Sarcosine Oxidase in Yeast Spore for Creatinine Degradation.
Appl Biochem Biotechnol
January 2025
School of Life Science and Bioengineering, Jining University, 1 Xingtan Road, Qufu, 273155, Shangdong, China.
Creatinine clearance is used to reflect the glomerular filtration rate to assess kidney function. Creatinine degradation-related enzymes have been used for creatinine detection in clinical medicine. The mixture of spores encapsulating either creatininase or creatinase or sarcosine oxidase could mediate a three-step reaction to produce hydrogen peroxide from creatinine.
View Article and Find Full Text PDFSelf-service aptamer-free molecularly imprinted paper-based sensor for high-sensitivity visual detection of influenza H5N1.
Analyst
January 2025
The key Laboratory for Green Organic Synthesis and Application of Hunan Province, College of Chemistry, Xiangtan University, Xiangtan, 411105, China.
Developing low-cost self-service portable sensors to detect viruses is an important step in combating the spread of viral outbreaks. Here, we describe the development of an aptamer-free paper-based molecularly imprinted sensor for the instrument-free detection of influenza virus A (H5N1). In this sensor, Whatman paper loaded with FeO nanoparticles (WP@FeO) was prepared as a substrate upon which silicon imprinting occurred in the presence of the template virus H5N1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!