A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days after flowering and obtained a maximum level at 16 days after flowering, the onset of overall protein accumulation. Significant expression of CAT activity in non-seed tissues occurred in some, but not all plants, suggesting possible chromosome position effects on non-seed tissue expression. A positive correlation was observed between expression levels in seeds and gene copy numbers.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF00015653 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ultrasound-assisted heat treatment: Accelerating rice glutelin fibrils formation and enhancing emulsifying properties.
Int J Biol Macromol
January 2025
College of Food Science and Engineering, Bohai University, National R&D Branch Center of Surimi and Surimi Products Processing, Jinzhou, Liaoning 121013, China. Electronic address:
The self-assembly of rice glutelin (RG) into RG fibrils (RGFs) represents a promising strategy for enhancing its functional properties. In this study, we investigated the effects of ultrasonic pretreatment on the fibrillation kinetics, structural characteristics, and functional properties of RGFs. The results indicated that ultrasonic pretreatment facilitated the unfolding of RG, resulting in an increased H and β-sheet, thereby accelerating the formation of RGFs and enhancing the fibril conversion rate.
View Article and Find Full Text PDFDifferent types of anions mediated the formation of rice glutelin fibrils: Aggregation behaviors and structural characteristics.
Food Chem
January 2025
School of Food Science and Technology, State Key Laboratory of Food Science and Resources, Jiangnan University, Lihu Road 1800, Wuxi 214122, China. Electronic address:
Anions have more pronounced effect on the aggregation power of proteins than cations. Herein, the effect of different types of anions on rice glutelin (RG) based fibrils formation was investigated. The fibrils yield and growth rate of RG were enhanced with various anions, due to the specific ions effect and intermolecular interaction.
View Article and Find Full Text PDFExtruded Quinoa Flour Applied for the Development of Gluten-Free Breads: a Technological, Sensory and Microstructural Approach.
Plant Foods Hum Nutr
January 2025
Laboratorio de Investigación en Funcionalidad y Tecnología de Alimentos (LIFTA), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Argentina.
Quinoa flour due to its nutritional and sensory characteristics could be used as an ingredient to improve the nutritional and technological properties of gluten-free bread. Furthermore, the application of hydrothermal processes such as extrusion can enhance their native properties. Hence, our objective was to evaluate how the incorporation of extruded quinoa flours (EQFs) affects the technological, sensory and nutritional quality of gluten-free bread.
View Article and Find Full Text PDFProduction of grains with low glutelin and high eating quality by using dominant allele Lgc-1 in three-line japonica hybrid rice.
Plant Biotechnol J
January 2025
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China.
Antiproliferative and antioxidant properties of protein-free and protein-bound phenolics isolated from purple rice (Oryza sativa L.).
Int J Biol Macromol
December 2024
National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China; Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, PR China. Electronic address:
Purple rice (Oryza sativa L.) is a rich in endogenous phenolics and proteins. The naturally occurring interactions between phenolic compounds and proteins have been shown to have beneficial effects on human health.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!