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Efficient production of a novel recombinant fusion protein of EIEC effector IpaD and EGFP: Biophysical characterization and functional studies.
Biochim Biophys Acta Proteins Proteom
March 2025
Department of Biotechnology, National Institute of Technology Durgapur, Durgapur, 713209, India. Electronic address:
The conserved invasion plasmid antigen D (IpaD) protein demonstrates broad protective capabilities against bacillary dysentery caused by Enteroinvasive Escherichia coli (EIEC) and Shigella. However, the instability of the IpaD protein at room temperature limits its therapeutic potential. The stabilization and efficient production of functional recombinant proteins remain critical challenges in therapeutic and vaccine development.
View Article and Find Full Text PDFTreatment-Related Mechanisms of Tibetan Medicine (TC) Aqueous Extract Against Mouse Gastroenteritis Caused by Yak-Origin Determined Using Intestinal Microbiome Analysis and Metabolomics.
Animals (Basel)
March 2025
College of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi 860000, China.
This study aimed to evaluate the therapeutic effect of (TC) on Tibetan yak-origin -induced diarrhea and dysentery in mice. The levels of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α), anti-inflammatory cytokines (IL-4 and IL-10), and the oxidative stress markers malondialdehyde (MDA), superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC), reduced glutathione (GSH-PX), and catalase (CAT) in the serum of mice were measured using ELISA kits. Using microbial diversity sequencing and non-targeted metabolomics detection techniques, the relevant mechanisms of TC treatment in a mouse infection model were evaluated.
View Article and Find Full Text PDFNetwork pharmacology combines cellular experiments to investigate the anti-inflammatory phytochemicals of vine of Pueraria montana var. lobata and their mechanism.
J Ethnopharmacol
March 2025
Jiangxi Province Key Laboratory of Pharmacology of Traditional Chinese Medicine, National Engineering Research Center for Modernization of Traditional Chinese Medicine - Hakka Medical Resources Branch, School of Pharmacy, Gannan Medical University, Ganzhou, Jiangxi, 341000, China. Electronic address:
Ethnopharmacological Relevance: Pueraria montana var. lobata (PM) has the effects of relieving muscle stiffness and fever, generating body fluids and quenching thirst, resolving rashes, raising yang and stopping diarrhea, unblocking meridians, and detoxifying alcohol. It is commonly used for the management of conditions including stiff neck and back pain, thirst, diabetes, unresolved measles, external fever with headache, dysentery, diarrhea, dizziness and headache, stroke with hemiplegia, chest and heart pain, and alcohol poisoning.
View Article and Find Full Text PDFEvaluating quality consistency of Celosiae Cristatae Flos by four dimensional fingerprints combined with antioxidant activity.
Spectrochim Acta A Mol Biomol Spectrosc
March 2025
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China. Electronic address:
Celosiae Cristatae Flos (CCF) has haemostatic and anti-dysentery properties, making it a natural medicine worthy of further development. However, there are few studies on it and most of them are based on high performance liquid chromatography. Consequently, there is a need to develop a comprehensive quality evaluation method based on multiple analytical techniques to reveal the composition of CCF.
View Article and Find Full Text PDFA Demographic Study of Aste-Béon (Ossau Valley, Bearn), a Small Community From French Western Pyrenees in the 19th Century.
Am J Hum Biol
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Laboratoire PACEA UMR CNRS 5199, Université de Bordeaux, Pessac, France.
Objectives: To decipher the social behaviors and public health status of a 19th-century small Western Pyrenean community using various historical demography data.
Methods: From censuses, civil and parish registers of births, marriages, and deaths/burials, migration archives, and administrative records, we analyzed a series of demographic parameters in the village of Aste-Béon between 1801 and 1900.
Results: Acknowledging the limitations of such a historical demography approach (especially losses of data and underreporting of deaths of very young individuals), this study enabled us to draw a series of conclusions.
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