Objectives: This study was designed to explore the function of ATP binding cassette transporter 1 (ABCA1) and Apolipoprotein A-I (ApoA-I) in cholesterol reverse transportation (RCT), the influence of lovastatin and rosiglitazone on the concentration of cholesterol (CHO) in THP-1 (human monocytic leukemia cell line) derived foam cells.
Methods: LDL from healthy volunteers was obtained by density-gradient ultracentrifugation and was oxidized by incubation with Cu2+ and ox-LDL was identified.Macrophages were induced from THP-1 cell by phorbol ester (PMA). Models of foam cells were built by incubating macrophages with oxLDL. The effect of lovastatin and rosiglitazone on ABCA1 protein expression in THP-1 cell line derived macrophage were detected by western blot. Foam cells were divided into 9 groups: control, ApoA-I, lovastatin, rosiglitazone lovastatin+ApoA-I, rosiglitazone+ApoA-I, ABCA1 monoclonal antibody pretreatment+ApoA-I, ABCA1 monoclonal antibody pretreatment+lovastatin+ApoA-I, ABCA1 monoclonal antibody pretreatment+rosiglitazone+ApoA-I. The concentration of intracellular CHO in each group was detected by using cholesterol kit.
Results: As compared with control group, there are no big differences of CHO concentration within the cell of group lovastatin, rosiglitazone, and each ABCA1 monoclonal antibody pretreatment group (P>0.05), but the CHO concentration within the cells of group ApoA-I, lovastatin+ApoA-I, rosiglitazone+ApoA-I decreased obviously as compared with the control (P<0.05), and CHO concentration in group rosiglitazone+ApoA-I have a further decrease than the former two groups (P<0.05).
Conclusions: CHO concentration can be decreased in foam cells by cooperation of ABCA1 and ApoA-I mediate cholesterol efflux. Rosiglitazone can enhance this procedure in THP-1 macrophages derived foam cells which means that they can promote ABCA1 mediated cholesterol reverse transportation through improve ABCA1 protein expression.
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