AI Article Synopsis
- Recent research indicates that soluble forms of amyloid β-protein (Aβ) might be responsible for synaptic issues seen in early Alzheimer's disease.
- There has been significant effort to isolate and study various Aβ assemblies to understand their role in the disease.
- This study specifically uses immunoprecipitation/western blotting and size exclusion chromatography/western blotting to analyze Aβ present in cell cultures, human cerebrospinal fluid, and samples from the human cortex.
Article Abstract
Recent data suggest that soluble, non-fibrillar assemblies of the amyloid β-protein (Aβ) may mediate the synaptic deficits that characterize the early stages of Alzheimer's disease. Consequently, much effort has been expended in isolating and studying a variety of different Aβ assemblies. Here, we describe the use of immunoprecipitation/western blotting and size exclusion chromatography/western blotting to characterize Aβ present in conditioned medium from cultured cells, human cerebrospinal fluid, and human cortex extracted with aqueous buffer, detergent, and formic acid.
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| http://dx.doi.org/10.1007/978-1-60761-744-0_3 | DOI Listing |
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