Objective: To investigate the molecular biological mechanism of chronoexercise regulating circadian.

Methods: Expressions of mPer1 and mPer2 in the diencephalon of golden hamster were determined 2 hours after acute exhaustive exercise (circadian time 6) by quantitative RT-PCR.

Results: Chronoexercise at CT6 significantly decreased expressions of mPer1 and mPer2 in the diencephalon of golden hamster.

Conclusion: Inhibitory effect of chronoexercise on Per1 and Per2 mRNA levels in the diencephalon of golden hamster at CT6 may be achieved transcription-translation-based autoregulatory negative feedback loop.

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