Contamination of an in-house diagnostic real-time PCR for Q fever was traced back to a commercially obtained PCR Master Mix. It was established that this Master Mix contained DNA from Coxiella burnetii, probably as a result of the use of compounds of animal origin such as bovine serum albumin.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008432 | PMC |
| http://dx.doi.org/10.1128/JCM.00464-10 | DOI Listing |
Publication Analysis
Top Keywords
master mix
12
pcr master
8
dna coxiella
8
coxiella burnetii
8
contamination commercial
4
commercial pcr
4
mix dna
4
burnetii contamination
4
contamination in-house
4
in-house diagnostic
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!