A liquid chromatographic/tandem mass spectrometric method using pneumatically assisted electrospray ionization (LC-ESI-MS/MS) was developed for determination of N-acetylneuramic acid and N-glycolylneuramic acid in infant formula. Reconstituted samples were hydrolysed in dilute sulfuric acid and deproteinized with acetonitrile. The extract was analysed directly without further clean-up by hydrophilic interaction chromatography. The substances were detected in negative ion mode and matrix matched standards were used for calibration. The relative intra-laboratory reproducibility standard deviation was better than 6% for both substances. An R² of 0.985 was obtained by comparison with a classical colorimetric assay based on reaction with resorcinol. The developed method is expected to be applied for accurate routine analysis of infant formulas.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/bmc.1429 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fecal Microbiome and Metabolomic Profiles of Mixed-Fed Infants Are More Similar to Formula-Fed than Breastfed Infants.
Microorganisms
January 2025
Department of Food Science & Human Nutrition, University of Illinois, Urbana, IL 61801, USA.
Many infants consume both human milk and infant formula (mixed-fed); however, few studies have investigated how mixed feeding affects the gut microbiome composition and metabolic profiles compared to exclusive breastfeeding or formula feeding. Herein, how delivery mode and early nutrition affect the microbiome and metabolome of 6-week-old infants in the STRONG Kids2 cohort was investigated. Fecal samples were collected from exclusively breastfed (BF; n = 25), formula-fed (FF; n = 25) or mixed-fed (MF; n = 25) participants.
View Article and Find Full Text PDFγ-Aminobutyric Acid (GABA) Metabolic Bypass Plays a Crucial Role in Stress Tolerance and Biofilm Formation in ATCC 29544.
Foods
January 2025
The College of Life and Geographic Sciences, Kashi University, Kashi 844000, China.
is a foodborne pathogen characterized by its robust stress tolerance and ability to form biofilms, which facilitates its survival in powdered infant formula (PIF) processing environments for prolonged periods. Gamma-aminobutyric acid (GABA) is a kind of non-protein amino acid that acts as an osmoprotectant. This study aimed to elucidate the effects of the gene on the survival of , GABA accumulation, and biofilm formation under desiccation, osmotic stress, and acid exposure.
View Article and Find Full Text PDFDietary fiber content in clinical ketogenic diets modifies the gut microbiome and seizure resistance in mice.
Nat Commun
January 2025
Department of Integrative Biology & Physiology, University of California, Los Angeles, CA, USA.
The gut microbiome modulates the anti-seizure effects of the ketogenic diet, but how specific dietary formulations differentially modify the gut microbiome in ways that impact seizure outcome is poorly understood. We find that medical ketogenic infant formulas vary in macronutrient ratio, fat source, and fiber content and differentially promote resistance to 6-Hz seizures in mice. Dietary fiber, rather than fat ratio or source, drives substantial metagenomic shifts in a model human infant microbial community.
View Article and Find Full Text PDFComparison of fluoride level between mother's milk and infant formula milk - a global systematic review and dental fluorosis risk assessment.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
January 2025
Department of Pediatrics and Neonatology, Guangzhou University of Traditional Chinese Medicine Dongguan Hospital, Guangdong Province, China.
Dental Fluorosis (DF) is one of the negative outcomes of excessive fluoride (F) intake through food sources. This systematic review aimed to compare F content in two important food sources for infants, Mother's Milk (MoM) and Infant Formula (IF), and then evaluate the risk of DF related to F in those two types of food. For this purpose, 181 studies were initially found by searching the relevant keywords in widely recognized databases, including Google Scholar, Scopus, Science Direct, and PubMed.
View Article and Find Full Text PDFAn Overview of Early-Life Gut Microbiota Modulation Strategies.
Ann Nutr Metab
January 2025
Department of Paediatrics, Medical University of Warsaw, Warsaw, Poland.
Background: The gut microbiota, or microbiome, is essential for human health. Early-life factors such as delivery mode, diet, and antibiotic use shape its composition, impacting both short- and long-term health outcomes. Dysbiosis, or alterations in the gut microbiota, is linked to conditions such as allergies, asthma, obesity, diabetes, inflammatory bowel disease, and necrotizing enterocolitis in preterm infants.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!