Quantitative analysis of mitochondrial DNA (mtDNA) and its common deletion (CD) are sensitive and early markers for mitochondrial mutations and suffering. However, the use of purified DNA can lead to quantification errors because of variable DNA extraction yields due to the significant differences in size and structure between genomic DNA (gDNA) and mtDNA. We report a real-time qPCR-based protocol directly on tissue lysate, without DNA extraction. This method, which allows both absolute and relative measure, increases the measuring accuracy of the mtDNA/gDNA ratio and leads to reliable and more reproducible results when measuring the deleted/total mtDNA ratio.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ab.2010.10.010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The complete mitochondrial genomes of and .
Mitochondrial DNA B Resour
January 2025
Department of Wildlife, Fisheries and Aquaculture, Mississippi State University, Mississippi State, Mississippi, USA.
We present a novel mitogenome assembly of the Redlip Shiner, , and assemblies for the Greenhead Shiner, (Cypriniformes: Leuciscidae). Both are charismatic minnows in the taxonomic group and are endemic to the eastern United States. The genome contains 16,711bp and 16,706bp each comprising a total of 13 protein coding genes, 22 tRNAs, two rRNAs, and a control region.
View Article and Find Full Text PDFStructural characterization and phylogenetic analysis of the chloroplast genome of var. balansae (gagnep.) N. Jacobsen 1991.
Mitochondrial DNA B Resour
January 2025
Department of Ecology and Institute of Hydrobiology, Jinan University, Guangzhou, China.
is a popular ornamental aquatic plant for aquarists, although only six species are found in China. Destruction of the natural habitats of for human activities has led to a decline in its numbers. In this report, we sequenced and annotated the chloroplast genome for the first time.
View Article and Find Full Text PDFThe landscape of rare mitochondrial DNA variants in sudden cardiac death: A potential role for ATP synthase.
Heliyon
January 2025
Unit of Medical Genetics, Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy.
Sudden cardiac death (SCD) is a major health concern, which can be the sign of a latent mitochondrial disease. However, mitochondrial DNA (mtDNA) contribution is largely unexplored in SCD at population level. Recently, mtDNA variants have been associated with congenital cardiopathy and higher risk of ischemic heart disease, suggesting them as potential risk factors also in SCD.
View Article and Find Full Text PDFMitochondrial genome of : features, RNA editing, and insights into male sterility.
Front Plant Sci
January 2025
Bio-resource Research and Utilization Joint Key Laboratory of Sichuan and Chongqing, Chongqing Institute of Medicinal Plant Cultivation, Nanchuan, Chongqing, China.
Introduction: Mitochondria are essential organelles that provide energy for plants. They are semi-autonomous, maternally inherited, and closely linked to cytoplasmic male sterility (CMS) in plants. , a widely used medicinal plant from the Caprifoliaceae family, is rich in chlorogenic acid (CGA) and its analogues, which are known for their antiviral and anticancer properties.
View Article and Find Full Text PDFEpigenetic Threads of Neurodegeneration: TFAM's Intricate Role in Mitochondrial Transcription.
CNS Neurol Disord Drug Targets
January 2025
Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysuru 570015. JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.
There is a myriad of activities that involve mitochondria that are crucial for maintaining cellular equilibrium and genetic stability. In the pathophysiology of neurodegenerative illnesses, mitochondrial transcription influences mitochondrial equilibrium, which in turn affects their biogenesis and integrity. Among the crucial proteins for keeping the genome in optimal repair is mitochondrial transcription factor A, more commonly termed TFAM.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!