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TCR transgenic clone selection guided by immune receptor analysis and single-cell RNA expression of polyclonal responders.
Elife
December 2024
Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.
Since the precursor frequency of naive T cells is extremely low, investigating the early steps of antigen-specific T cell activation is challenging. To overcome this detection problem, adoptive transfer of a cohort of T cells purified from T cell receptor (TCR) transgenic donors has been extensively used but is not readily available for emerging pathogens. Constructing TCR transgenic mice from T cell hybridomas is a labor-intensive and sometimes erratic process, since the best clones are selected based on antigen-induced CD69 upregulation or IL-2 production in vitro, and TCR chains are polymerase chain reaction (PCR)-cloned into expression vectors.
View Article and Find Full Text PDFHigh-flow nasal cannula for pre- and apneic oxygenation during rapid sequence induction intubation in emergency surgery: A systematic review and meta-analysis.
PLoS One
January 2025
Department of Anesthesiology, The Second Affiliated Hospital, The Army Military Medical University, Chongqing, China.
Background: Rapid sequence induction intubation (RSII) is commonly used in emergency surgeries for patients at high risk of aspiration. However, these patients are more susceptible to hypoxemia during the RSII process. High-flow nasal cannula (HFNC) oxygen therapy has emerged as a potential alternative to traditional face mask (FM) ventilation pre- and apneic oxygenation.
View Article and Find Full Text PDFA Chromosome-Level Genome Sequence Reveals Regulation of Salt Stress Response in Mesembryanthemum crystallinum.
Physiol Plant
January 2025
Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan.
Salt stress disturbs plant growth and photosynthesis due to its toxicity. The ice plant Mesembryanthemum crystallinum is a highly salt-tolerant facultative crassulacean acid metabolism (CAM) plant. However, the genetic basis of the salt tolerance mechanisms in ice plants remains unclear.
View Article and Find Full Text PDFHennegoides Africanus sp. nov. from Kadey River in Cameroon.
Acta Parasitol
January 2025
Laboratory of Parasitology and Ecology, Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
Purpose: Fish are susceptible to various parasitic infections, with Myxozoa emerging as a major group. A taxonomic study of Myxozoa is essential for the rapid diagnosis of species potentially responsible for epizootic diseases.
Methods: The studied fish was collected from the Kadey River, a tributary of the Sangha River in the Congo Basin in Cameroon, and parasitologically dissected.
Non-invasive ventilation as a pre-oxygenation strategy during in-flight rapid sequence intubation: A case report.
Prehosp Emerg Care
January 2025
Emergency Medicine, Methodist Dallas Medical Center, Dallas, Texas.
Non-invasive ventilation has been used as a pre-oxygenation strategy for rapid sequence intubation in the emergency department and the intensive care unit, yet, limited research has examined its use in the transport setting. These case reports discuss the use of non-invasive ventilation via a Hamilton T1 ventilator (Hamilton Medical) during transport by an air medical crew for pre-oxygenation before intubation in two cases. In both cases, a non-invasive, bilevel-positive airway pressure mode with a backup rate was used to achieve adequate airway pressures while allowing for a two-handed seal by one EMS clinician as the other prepared the equipment and medications.
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