AI Article Synopsis
- Avian nephritis virus (ANV) was previously thought not to exist in Australia, but the first confirmed case has been identified in young chickens exhibiting dehydration and diarrhea.
- Histopathological analysis revealed interstitial nephritis, and while some vaccines for infectious bronchitis virus were present, they weren't the cause of the symptoms.
- Testing with specially designed PCR primers showed that six out of seven new cases, along with one historical case, tested positive for ANV, confirming its presence in Australian poultry flocks.
Article Abstract
Avian nephritis virus (ANV) is thought to infect poultry flocks worldwide, but no confirmed case has been reported in Australia. The first such case is described in this study. Cases of young chickens with clinical signs of dehydration and diarrhea were submitted to our laboratory and histopathology detected interstitial nephritis. Vaccine strains of infectious bronchitis virus were detected in some of these cases but were not considered to be the causative agent. A total of seven fresh submissions from broiler chicken flocks were collected at 8-11 days of age. Degenerate PCR primers were designed based on published ANV polymerase gene sequences and used to analyze historic cases as well as the fresh submissions. Six of the seven fresh submissions, and one historic case, were positive for ANV with nucleotide sequencing confirming these results. These results establish ANV as an infectious pathogen circulating in Australian poultry.
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| http://dx.doi.org/10.1637/9230-010610-Reg.1 | DOI Listing |
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