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http://dx.doi.org/10.1177/0883073810375118 | DOI Listing |
Neurol Clin Pract
June 2014
Washington University, St. Louis, MO.
PLoS One
September 2014
Department of Statistics, Iowa State University, Ames, Iowa, United States of America.
A central goal of RNA sequencing (RNA-seq) experiments is to detect differentially expressed genes. In the ubiquitous negative binomial model for RNA-seq data, each gene is given a dispersion parameter, and correctly estimating these dispersion parameters is vital to detecting differential expression. Since the dispersions control the variances of the gene counts, underestimation may lead to false discovery, while overestimation may lower the rate of true detection.
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