AI Article Synopsis
- The Yaba-like disease viruses (YLDV) are part of the Yatapoxvirus family and have DNA genomes crucial for understanding their pathology.
- The E3L protein from these viruses features two important domains for binding DNA and RNA, which play a role in their ability to cause disease.
- NMR hydrogen exchange experiments showed that the YLDV E3L protein can convert B-form DNA into left-handed Z-DNA, similar to how another protein (hZα(ADAR1)) achieves this transformation.
Article Abstract
The Yaba-like disease viruses (YLDV) are members of the Yatapoxvirus family and have double-stranded DNA genomes. The E3L protein, which is essential for pathogenesis in the vaccinia virus, consists of two domains: an N-terminal Z-DNA binding domain and a C-terminal RNA binding domain. The crystal structure of the E3L orthologue of YLDV (yabZα(E3L)) bound to Z-DNA revealed that the overall structure of yabZα(E3L) and its interaction with Z-DNA are very similar to those of hZα(ADAR1). Here we have performed NMR hydrogen exchange experiments on the complexes between yabZα(E3L) and d(CGCGCG)(2) with a variety of protein-to-DNA molar ratios. This study revealed that yabZα(E3L) could efficiently change the B-form helix of the d(CGCGCG)(2) to left-handed Z-DNA via the active-mono B-Z transition pathway like hZα(ADAR1).
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| http://dx.doi.org/10.1016/j.febslet.2010.10.003 | DOI Listing |
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