Arabidopsis thaliana peptide deformylase PDF1B was expressed in tobacco chloroplasts using spectinomycin as the selective agent. The foreign protein accumulated in chloroplasts (6% of the total soluble protein) and was enzymatically active. Transplastomic plants were evaluated for resistance to the peptide deformylase inhibitor actinonin. In vitro seed germination in the presence of actinonin and in planta application of the inhibitor demonstrated the resistance of the transformed plants. In addition, transgenic leaf explants were able to develop shoots via organogenesis in the presence of actinonin. However, when the combination of the PDF1B gene and actinonin was used as the primary selective marker system for chloroplast transformation of tobacco, all developed shoots were escapes. Therefore, under the experimental conditions tested, the use of this system for plastid transformation would be limited to function as a secondary selective marker.
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http://dx.doi.org/10.1007/s11248-010-9447-9 | DOI Listing |
BMC Microbiol
November 2024
Botany and Microbiology Department, Faculty of Science, Helwan University, Helwan, Egypt.
Proc Natl Acad Sci U S A
November 2024
Department of Biology, University of Rochester, Rochester, NY 14627.
Within a cell, protein-bound methionines can be chemically or enzymatically oxidized, and subsequently reduced by methionine sulfoxide reductases (Msrs). Methionine oxidation can result in structural damage or be the basis of functional regulation of enzymes. In addition to participating in redox reactions, methionines play an important role as the initiator residue of translated proteins where they are commonly modified at their α-amine group by formylation or acetylation.
View Article and Find Full Text PDFOrg Biomol Chem
August 2024
Department of Pharmacy, Banasthali Vidyapith, Banasthali, India.
A base-promoted palladium-catalyzed cascade reaction is described to access trifluoromethylated dipyridodiazepinone derivatives in an aqueous system (1,4-dioxane-HO). This methodology uses simple chemicals, has a broad substrate scope, is waste minimized (-factor = 0.3-0.
View Article and Find Full Text PDFFront Chem
June 2024
Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research, Kolkata, West Bengal, India.
In this study, 1,4-benzothiazine-based bisamide derivatives, a new class of antibacterial agents targeting bacterial peptide deformylase (PDF), were designed and synthesized to combat infection. Molecular modeling of the designed molecules showed better docking scores compared to the natural product actinonin. Bioactivity assessment identified two derivatives with promising antibacterial activity .
View Article and Find Full Text PDFArch Biochem Biophys
August 2024
Department of Biological Science & Engineering, MANIT, Bhopal, India. Electronic address:
Streptococcus oralis an opportunistic bacterium has been reported to be involved in various blood borne infections like subacute bacterial endocarditis, septicemia, bacterial meningitis and in some cases dental caries too. Among various targets the peptide deformylase, of S.oralis appears to be most potent druggable target as it is involved in protein synthesis is opted for the current study.
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