Mycothiol (MSH) was reported to be the dominant low molecular weight thiol in members of the Actinobacteria. In this study, a simple, fast, and sensitive method for qualitative and quantitative determination of MSH molecules was developed based on maleylpyruvate isomerase (MPI) from Corynebacterium glutamicum. The principle of this method is that the activity of MPI from C. glutamicum was dependent on MSH molecules. It was found that this MPI activity displayed a linear response (R (2) = 0.9928) at MSH amounts ranging from 0.12 to 3.98 pmol in the defined assay system. This observation was applied to calculate the MSH levels, and the newly developed method was compared with thiol-specific fluorescent-labeling high-performance liquid chromatography method. Forty-eight genera of Actinobacteria were screened for MSH and 43 genera were reported for MSH occurrence, and the MSH levels in Actinobacteria were determined to be 0.01 to 9.69 μmol/g of residual dry cell weight.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00253-010-2918-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Revisiting the electrophysiological correlates of valence and expectancy in reward processing - A multi-lab replication.
Cortex
January 2025
Department of Experimental Clinical & Health Psychology, Ghent University, Ghent, Belgium.
Two event-related brain potential (ERP) components, the frontocentral feedback-related negativity (FRN) and the posterior P300, are key in feedback processing. The FRN typically exhibits greater amplitude in response to negative and unexpected outcomes, whereas the P300 is generally more pronounced for positive outcomes. In an influential ERP study, Hajcak et al.
View Article and Find Full Text PDFEvaluation of Outcomes Following Arthroscopic Treatment of Femoroacetabular Impingement Syndrome Across Different Surgeons, Surgical Techniques, and Postoperative Protocols: A Multi-institutional Study.
Orthop J Sports Med
January 2025
Department of Orthopaedic Surgery and Sports Medicine, University of Washington, Seattle, Washington, USA.
Background: Femoroacetabular impingement syndrome (FAIS) is frequently treated arthroscopically with osteoplasty and labral repair. Surgical preferences vary in terms of equipment, technique, and postoperative protocol. Patient-reported outcome measures (PROMs) are valuable tools to assess outcomes across different institutions.
View Article and Find Full Text PDFBalancing act: optimizing blue light for melanogenesis while minimizing cellular damage in primary human skin cells.
Front Physiol
January 2025
Regenerative Medicine Division, CHU de Quebec - Université Laval Research Centre, Quebec City, QC, Canada.
Introduction: Recent findings show that visible light, particularly blue light, stimulates melanogenesis in human skin, though the underlying mechanisms remain debated. This study aimed to determine the cell damage threshold of non-ionizing blue light on keratinocytes while preserving their ability to stimulate melanogenesis.
Methods: Human keratinocytes (N = 3) and melanocytes (N = 3) were isolated from skin samples of varying Fitzpatrick skin phototypes and irradiated with blue light (λpeak = 457 nm) and UVA light (λpeak = 385 nm).
Fractionation of High-Yield Noncondensed Lignin and Glucan Oligomers from Lignocellulose in a Novel Biphasic System.
J Agric Food Chem
January 2025
Guangdong University of Technology, Guangzhou 510006, China.
Effective fractionation of lignocellulose into hemicellulose, cellulose, and lignin is the precondition for full-component valorization. Generally, harsh reaction conditions are used to improve fractionation efficiency, which leads to severe lignin condensation and inhibits its value-added applications. To address this issue, a novel biphasic system consisting of molten salt hydrates (MSHs) and n-butanol was developed for birch fractionation.
View Article and Find Full Text PDFNovel Pharmacologic Treatments of Female Sexual Dysfunction.
Clin Obstet Gynecol
March 2025
Department of Psychiatry & Neurobehavioral Sciences.
This review evaluates pharmacologic treatments for female sexual dysfunction (FSD), focusing on hypoactive sexual desire disorder (HSDD). We provide clinically relevant applications for Food and Drug Administration (FDA)-approved medications (flibanserin and bremelanotide) and investigational therapies (Lorexys and testosterone combinations). Detailed study outcomes, safety profiles, and clinical strategies guide clinicians in appropriate diagnosis, patient selection, expectation setting, side effect management, and patient education, improving treatment outcomes and patient satisfaction.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!