Liquid crystal cubic phase formed with monoolein has been used as immobilizing matrix to host redox protein hemoglobin on glassy carbon electrode surface. The promoted direct electron transfer between hemoglobin and electrode was observed and a large average kinetic electron transfer rate constant k(s) of 3.03(±0.02)s(-1) was estimated. The electrode modified with cubic phase containing hemoglobin retains the bioactivity of hemoglobin and shows excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide with a small apparent Michaelis-Menten constant of 0.25(±0.03)mM. A novel reagentless hydrogen peroxide biosensor was constructed using the hemoglobin-containing cubic phase modified electrode and the proposed hydrogen peroxide biosensor shows a linear range of 7.0-239μM with a detection limit of 3.1(±0.2)μM and good stability and reproducibility.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.colsurfb.2010.09.009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Passion fruit seed extract protects hydrogen peroxide-induced cell damage in human retinal pigment epithelium ARPE-19 cells.
Sci Rep
January 2025
Department of Biochemistry and Molecular Biology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
Age-related macular degeneration (AMD) is a major cause of vision loss among adults. We investigated the protective effects of passion fruit seed extract (PFSE) and its rich polyphenol piceatannol in an AMD cell model in which human retinal pigment epithelial ARPE-19 cells were exposed to hydrogen peroxide (HO). Using a cell viability WST-8 assay, we revealed that PFSE and piceatannol increased the cellular viability of ARPE-19 cells by 130% and 133%, respectively.
View Article and Find Full Text PDFInhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice.
Nat Commun
January 2025
NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, The province and ministry co-sponsored collaborative innovation center for medical epigenetics, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, 300134, China.
Reactive oxygen species exacerbate nonalcoholic steatohepatitis (NASH) by oxidizing macromolecules; yet how they promote NASH remains poorly understood. Here, we show that peroxidase activity of global hepatic peroxiredoxin (PRDX) is significantly decreased in NASH, and palmitic acid (PA) binds to PRDX1 and inhibits its peroxidase activity. Using three genetic models, we demonstrate that hepatic PRDX1 protects against NASH in male mice.
View Article and Find Full Text PDFHighly sensitive photoluminescence sensor based on chitosan biopolymer film for determination of hydrogen peroxide.
Int J Biol Macromol
January 2025
Department of Statistics, Imam Khomeini International University, Qazvin, Iran.
Determination of hydrogen peroxide (HO) is of great importance in many systems for controlling the quality of products, food safety, and medical diagnostics. In this work, a highly sensitive photoluminescence film sensor was synthesized based on chitosan (CS), polyvinyl alcohol (PVA), and terephthalic acid (TPA), in the presence of copper (II) ions for determination of hydrogen peroxide. TPA was used as a sensitive probe for detection of hydroxyl radicals produced in a photo-Fenton-like process.
View Article and Find Full Text PDFHigher concentration of trehalose dihydrate stabilizes recombinant IgG1 under forced stress conditions.
J Pharm Sci
January 2025
Department of Chemical Engineering, Indian Institute of Technology Delhi. Electronic address:
Stability of complex biotherapeutics like monoclonal antibodies is paramount for their safe and efficacious use. Excipients are inactive ingredients that are added to the purified product so as to offer it a stable environment. Trehalose dihydrate is a non-reducing sugar that is commonly used as a stabilizing agent in biotherapeutic formulations under liquid and frozen states.
View Article and Find Full Text PDFApplication of the composite nanoparticles of selenium and chitosan for ameliorating arsenic stress in rice seedlings.
Plant Physiol Biochem
January 2025
Department of Botany, Cotton University, Guwahati, 781001, Assam, India. Electronic address:
Selenium nanoparticles are well known for their antioxidant and stress-mitigating properties. In our study, composite nanoformulations of selenium and chitosan have been synthesized. The synthesized composite nanoformulations were 50 nm in diameter, spherical in shape, and had higher antioxidant activities and stability than the selenium and chitosan nanoparticles.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!