AI Article Synopsis
- Researchers examined MHC class I alleles in Mauritian cynomolgus macaques using high-resolution pyrosequencing to better understand disease susceptibility and immunity.
- The study identified 67 unique MHC class I transcripts from the seven most common haplotypes, with 40 of them covering full gene sequences.
- This new method has greatly increased the known MHC diversity in these primates and opens up possibilities for future research on diseases and immune responses.
Article Abstract
Major histocompatibility complex (MHC) class I alleles of nonhuman primates have been associated with disease susceptibility, resistance, and resolution. Here, using high-resolution pyrosequencing, we characterized MHC class I transcripts expressed in Mauritian cynomolgus macaques (MCM), a nonhuman primate population with restricted MHC diversity. Using this approach, we identified 67 distinct MHC class I transcripts encoded by the seven most frequent MCM MHC class I haplotypes, 40 (60%) of which span the complete open reading frames. These results double the number of MHC class I sequences previously defined by cloning and Sanger sequencing of cDNA-PCR products and provide a rapid, high-throughput, and economical method for MHC characterization. Overall, this approach significantly expanded our knowledge of MCM haplotypes and will facilitate future studies on disease pathogenesis and protective cellular immunity.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3077881 | PMC |
| http://dx.doi.org/10.1007/s00251-010-0481-9 | DOI Listing |
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