Plant shoots have thick, polylamellate outer epidermal walls based on crossed layers of cellulose microfibrils, but the involvement of microtubules in such wall lamellation is unclear. Recently, using a long-term movie system in which Arabidopsis seedlings were grown in a biochamber, the tracks along which cortical microtubules move were shown to undergo slow rotary movements over the outer surface of hypocotyl epidermal cells. Because microtubules are known to guide cellulose synthases over the short term, we hypothesised that this previously unsuspected microtubule rotation could, over the longer term, help explain the cross-ply structure of the outer epidermal wall. Here, we test that hypothesis using Arabidopsis plants expressing the cellulose synthase GFP-CESA3 and show that cellulose synthase trajectories do rotate over several hours. Neither microtubule-stabilising taxol nor microtubule-depolymerising oryzalin affected the linear rate of GFP-CESA3 movement, but both stopped the rotation of cellulose synthase tracks. Transmission electron microscopy revealed that drug-induced suppression of rotation alters the lamellation pattern, resulting in a thick monotonous wall layer. We conclude that microtubule rotation, rather than any hypothetical mechanism for wall self-assembly, has an essential role in developing cross-ply wall texture.
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ISME Commun
January 2025
State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China.
Antarctic snow harbors diverse microorganisms, including pigmented algae and bacteria, which create colored snow patches and influence global climate and biogeochemical cycles. However, the genomic diversity and metabolic potential of colored snow remain poorly understood. We conducted a genome-resolved study of microbiomes in colored snow from 13 patches (7 green and 6 red) on the Fildes Peninsula, Antarctica.
View Article and Find Full Text PDFMar Environ Res
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College of Marine Technology and Environment, Dalian Ocean University, Dalian, 116023, PR China; Key Laboratory of Marine Bio-Resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian, 116023, PR China. Electronic address:
Suaeda salsa, the dominant herbaceous plant in the high salinity areas of Asia, can even grow in the heavy metal polluted region. In order to illustrate the mechanisms of Cd (cadmium) tolerance in S. salsa, the accumulation, physiological and proteomic characters under two different concentrations of Cd exposure were investigated in this study.
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Department of Agronomy, Horticulture, and Plant Science, South Dakota State University (SDSU), Brookings, SD, 57007, USA.
Background: Hexaploid oat (Avena sativa L.) is a commercially important cereal crop due to its soluble dietary fiber β-glucan, a hemicellulose known to prevent cardio-vascular diseases. To maximize health benefits associated with the consumption of oat-based food products, breeding efforts have aimed at increasing the β-glucan content in oat groats.
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Department of Molecular Biology and Genetics, Ordu University, Ordu, Turkey.
Int J Biol Macromol
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Institute of Qinghai-Tibetan Plateau, Southwest Minzu University, Chengdu, Sichuan 610225, PR China; Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory, Southwest Minzu University, Chengdu, Sichuan 610225, PR China. Electronic address:
Cellulose, synthesized by cellulose synthase (CESA) complexes, is an essential component of plant cell walls; defects in cellulose synthesis compromise cell wall integrity. The maintenance of this integrity is vital for plant growth, development, and stress responses. Consequently, plants must continuously synthesize and remodel their cell walls, a process intricately linked to cellulose biosynthesis.
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