AI Article Synopsis
- The Zα domain of human ADAR1 has a strong preference for binding to Z-DNA over B-DNA, indicating a specific affinity for certain DNA conformations.
- It binds to Z-conformational non-CG-repeat DNA duplexes and shows consistent behavior with the d(CGCGCG)(2) duplex.
- NMR experiments reveal that Zα(ADAR1) exhibits a sequence preference during the B-Z transition, with a ranking of d(CGCGCG)(2) being the most favored, followed by d(CACGTG)(2) and d(CGTACG)(2).
Article Abstract
The Zα domain of human ADAR1 (Zα(ADAR1)) preferentially binds Z-DNA rather than B-DNA with high binding affinity. Zα(ADAR1) binds to the Z-conformation of both non-CG-repeat DNA duplexes and a d(CGCGCG)(2) duplex similarly. We performed NMR experiments on complexes between the Zα(ADAR1) and non-CG-repeat DNA duplexes, d(CACGTG)(2) or d(CGTACG)(2), with a variety of protein-DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG)(2) in the previous study suggests that Zα(ADAR1) exhibits the sequence preference of d(CGCGCG)(2)≫d(CACGTG)(2)>d(CGTACG)(2) through multiple sequence discrimination steps during the B-Z transition.
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| http://dx.doi.org/10.1016/j.febslet.2010.09.036 | DOI Listing |
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