Miniaturization of the classical test tube to picoliter dimensions is possible in monodisperse water-in-oil droplets that are generated in microfluidic devices. The establishment of standard unit operations for droplet handling and the ability to carry out experiments with DNA, proteins, cells and organisms provides the basis for the design of more complex workflows to address biological challenges. The emerging experimental format makes possible a quantitative readout for large numbers of experiments with a precision comparable to the macroscopic scale. Directed evolution, diagnostics and compound screening are areas in which the first steps are being taken toward the long-term goal of transforming the way we design and carry out experiments.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cbpa.2010.08.013 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nasal food challenge with hen's egg white allergen.
Allergy Asthma Clin Immunol
January 2025
Department of Prevention of Environmental Hazards, Allergology and Immunology, Medical University of Warsaw, Zwirki I Wigury 61, 02-097, Warsaw, Poland.
Background: Nasal allergen provocation tests are an important part of the diagnostics of allergic diseases triggered by environmental factors. Recently, increased attention has been paid to the potential use of this method in the diagnosis of food allergy. The objective of the study was to evaluate the usefulness of the nasal allergen provocation test in a group of subjects allergic to hen's egg white allergens.
View Article and Find Full Text PDFFast, bioluminescent blinks attract group members of the nocturnal flashlight fish Anomalops katoptron (Bleeker, 1856).
Front Zool
January 2025
Department of General Zoology and Neurobiology, Institute of Biology and Biotechnology, Ruhr-University Bochum, 44801, Bochum, Germany.
Background: During their nighttime shoaling, the flashlight fish Anomalops katoptron produce fascinating, bioluminescent blink patterns, which have been related to the localization of food, determination of nearest neighbor distance, and initiation of the shoal's movement direction. Information transfer e.g.
View Article and Find Full Text PDFGibberellin-3 induced dormancy and suppression of flower bud formation in pitaya (Hylocereus polyrhizus).
BMC Plant Biol
January 2025
Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
Background: Flowering is a complex, finely regulated process involving multiple phytohormones and transcription factors. However, flowering regulation in pitaya (Hylocereus polyrhizus) remains largely unexamined. This study addresses this gap by investigating gibberellin-3 (GA3) effects on flower bud (FB) development in pitaya.
View Article and Find Full Text PDF5,7-Dihydroxy-4-Methylcoumarin enhances osteogenesis and ameliorates osteoporosis via the AKT1 pathway.
Biochem Pharmacol
January 2025
School of Medicine, Nankai University, Tianjin, PR China. Electronic address:
Osteoporosis is a chronic disease distinguished by decreased bone density and degradation of bone microstructure, frequently linked with inflammation and oxidative stress, both of which contribute to the acceleration of bone resorption. The compound 5,7-Dihydroxy-4-methylcoumarin (D4M) present in Artemisia dracunculus exhibits significant antioxidant and anti-inflammatory properties. Nonetheless, the potential anti-osteoporotic effects of D4M, along with the molecular targets and mechanisms responsible for these effects, have not been studied.
View Article and Find Full Text PDFA Mutant Complement Factor H (W1183R) Enhances Proteolytic Cleavage of von Willebrand Factor by ADAMTS13 Under Shear.
J Thromb Haemost
January 2025
Department of Pathology and Laboratory Medicine; Institute of Reproductive Medicine and Developmental Sciences, The University of Kansas Medical Center, Kansas City, KS 66160. Electronic address:
Background: A loss-of-functional mutation (W1183R) in human complement factor H (CFH) is associated with complement-associated hemolytic uremic syndrome; mice carrying a similar mutation (W1206R) in CFH also develop thrombotic microangiopathy but its plasma von Willebrand factor (VWF) multimer sizes were dramatically reduced. The mechanism underlying such a dramatic change in plasma VWF multimer distribution in these mice is not fully understood.
Objective And Methods: To determine the VWF and CFH interaction and how CFH proteins affect VWF multimer distribution, we employed recombinant protein expression, purification, and various biochemical and biophysical tools.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!