AI Article Synopsis
- A new method was developed to measure 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in cell culture fluids using solid-phase extraction and HPLC-ECD.
- The researchers used a technique that adds known amounts of 8-oxodG to counteract interference from the complex cell growth medium.
- After applying this method to X-ray irradiated fibroblasts, they found that radiation increased 8-oxodG levels significantly, indicating it could serve as a biomarker for oxidative stress from moderate X-ray exposure.*
Article Abstract
We have developed a simple methodology, based on single-step solid-phase extraction followed by isocratic high-performance liquid chromatography coupled with electrochemical detection (HPLC-ECD), to determine extracellular 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in culture supernatants of normal human dermal fibroblasts. A standard addition method, using externally added 8-oxodG (0.5 and 1 pmol) was employed to eliminate matrix effects arising from the chemically complex, protein-rich medium. Secondly, applying this procedure to X-ray irradiated fibroblasts, we report a significant twofold increase in the levels of 8-oxodG at the radiobiologically relevant dose of 6 Gy. This suggests that extracellular 8-oxodG might be a useful biomarker for oxidative stress following moderate doses of X-irradiation.
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| http://dx.doi.org/10.3109/1354750X.2010.511269 | DOI Listing |
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