Objective: To evaluate sperm DNA fragmentation and semen parameters to diagnose male factor infertility and predict pregnancy after IVF.
Design: Prospective study.
Setting: Academic research laboratory.
Patient(s): Seventy-five couples undergoing IVF and 28 fertile donors.
Intervention(s): Sperm DNA fragmentation was measured by the alkaline Comet assay in semen and sperm after density gradient centrifugation (DGC). Binary logistic regression was used to analyze odds ratios (OR) and relative risks (RR) for IVF outcomes.
Main Outcome Measure(s): Semen parameters and sperm DNA fragmentation in semen and DGC sperm compared with fertilization rates, embryo quality, and pregnancy.
Result(s): Men with sperm DNA fragmentation at more than a diagnostic threshold of 25% had a high risk of infertility (OR: 117.33, 95% confidence interval [CI]: 12.72-2,731.84, RR: 8.75). Fertilization rates and embryo quality decreased as sperm DNA fragmentation increased in semen and DGC sperm. The risk of failure to achieve a pregnancy increased when sperm DNA fragmentation exceeded a prognostic threshold value of 52% for semen (OR: 76.00, CI: 8.69-1,714.44, RR: 4.75) and 42% for DGC sperm (OR: 24.18, CI: 2.89-522.34, RR: 2.16).
Conclusion(s): Sperm DNA testing by the alkaline Comet assay is useful for both diagnosis of male factor infertility and prediction of IVF outcome.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fertnstert.2010.08.019 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Protective effects of Mito-TEMPO on ischemia-reperfusion injury in a mouse testicular torsion and detorsion model.
Vet Res Forum
December 2024
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Testicular ischemia-reperfusion (I/R) injury during testicular torsion is strongly influenced by oxidative stress caused by excessive accumulation of unscavenged reactive oxygen species. This study aimed to investigate the effects of intra-peritoneal administration of Mito-TEMPO (MT) on I/R injury in testicular torsion/detorsion (T/D) in mice. Forty-two male mice were divided into seven groups including 1 control and 6 treatment groups (360° T/D, 720° T/D, 360° T/D + 0.
View Article and Find Full Text PDFThe involvements of intracellular basal calcium and membrane potential in para-phenylenediamine-impaired sperm function.
Transl Androl Urol
December 2024
Center for Reproductive Medicine, Affiliated Hospital of Nantong University, Nantong, China.
Background: Para-phenylenediamine (PPD) is a crystalline solid that belongs to the aromatic amine group, widely used in the manufacturing of various dyes. PPD exhibits toxic effects on female hormone stability, ovarian function, and embryo development. Although studies have shown that PPD exposure can damage oocyte quality in female mice, research on its effects on male reproductive capability, particularly on human sperm quality and function, is limited.
View Article and Find Full Text PDFSustanon suppresses spermatogenesis and increases cell death.
Transl Androl Urol
December 2024
Department of Pathology, Pediatric and Perinatal Pathology, University of Miami Miller School of Medicine, Miami, FL, USA.
Background: Anabolic-androgenic steroids (AAS) are synthetic derivatives of testosterone. Sustanon, dissolved in peanut oil, is an AAS used by athletes to build muscle mass. This study aims to examine the effects of Sustanon on male reproductive health.
View Article and Find Full Text PDFAcquired sperm hypomethylation by gestational arsenic exposure is re-established in both the paternal and maternal genomes of post-epigenetic reprogramming embryos.
Epigenetics Chromatin
January 2025
Department of Maternal‑Fetal Biology, National Center for Child Health and Development, Tokyo, 157‑8535, Japan.
Background: DNA methylation plays a crucial role in mammalian development. While methylome changes acquired in the parental genomes are believed to be erased by epigenetic reprogramming, accumulating evidence suggests that methylome changes in sperm caused by environmental factors are involved in the disease phenotypes of the offspring. These findings imply that acquired sperm methylome changes are transferred to the embryo after epigenetic reprogramming.
View Article and Find Full Text PDFShort and long-term effects of experimental varicocele.
Andrology
January 2025
Department of Surgery, Division of Urology, Human Reproduction Section, São Paulo Federal University, São Paulo, Brazil.
Background: Varicocele is associated with a progressive decrease in male fertile potential, but it has yet to be determined if the duration of varicocele is associated with altered sperm functional quality.
Objectives: This experimental study investigated the time-dependent effects of varicocele on spermatogenesis, sperm parameters, and sperm functional traits.
Materials And Methods: Thirty-five mature male Wistar rats (200 ± 25 g) were included.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!