AI Article Synopsis
- Researchers used micro-magnetic resonance imaging (μMRI) to observe Japanese quail embryos from Day 0 to 8, capturing detailed images of the embryos and surrounding structures.
- Significant growth occurred between Day 4 and Day 5, with the embryo length increasing by 43% and volume nearly tripling, alongside changes in yolk and albumen levels.
- The study confirmed that μMRI is an effective method for monitoring live avian embryo development, with no significant differences noted in embryos exposed to magnetic fields compared to control embryos.
Article Abstract
Eggs containing live Japanese quail embryos were imaged using micro-magnetic resonance imaging (μMRI) at 24-h intervals from Day 0 to 8, the period during which the main body axis is being laid down and organogenesis is taking place. Considerable detail of non-embryonic structures such as the latebra was revealed at early stages but the embryo could only be visualized around Day 3. Three-dimensional (3D) changes in embryo length and volume were quantified and also changes in volume in the extra- and non-embryonic components. The embryo increased in length by 43% and nearly trebled in volume between Day 4 and Day 5. Although the amount of yolk remained fairly constant over the first 5 days, the amount of albumen decreases significantly and was replaced by extra-embryonic fluid (EEF). ¹H longitudinal (T₁) and transverse (T₂) relaxation times of different regions within the eggs were determined over the first 6 days of development. The T₂ measurements mirrored the changes in image intensity observed, which can be related to the aqueous protein concentrations. In addition, a comparison of the development of Day 0 to 3 quail embryos exposed to radiofrequency (rf) pulses, 7 T static magnetic fields and magnetic field gradients for an average of 7 h with the development of control embryos did not reveal any gross changes, thus confirming that μMRI is a suitable tool for following the development of live avian embryos over time from the earliest stages.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3006493 | PMC |
| http://dx.doi.org/10.1016/j.mri.2010.08.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ovalbumin gene polymorphism: Implications for hatchability and egg quality changes during storage in Japanese quail.
Poult Sci
January 2025
Institute of Biological Bases of Animal Production, University of Life Sciences in Lublin, 13 Akademicka St., 20-950 Lublin, Poland.
The aim of the study was to identify polymorphisms in the ovalbumin gene - SERPINB14 gene and evaluate their effect on hatchability traits and egg quality changes during storage in two strains of Japanese quails: meat-type (F33) and laying-type (S22). To individually determine hatchability traits for each female, eggs were collected and incubated. To determine egg quality traits, 10 eggs were collected from each female and stored for 14 weeks.
View Article and Find Full Text PDFNew Findings in Developmental Studies of Laryngeal Mound With a Reference to Its Attached Structures in Japanese Quail (Coturnix coturnix Japonica).
Microsc Res Tech
January 2025
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
The laryngeal mound (LM) formed the caudal part of the pharyngeal floor, which varied in position, shape, and length at different ages. This work aimed to study the morphogenesis of the LM in the embryonic and post hatching periods grossly, histologically, and by scanning electron microscopy using forty-eight Japanese quails. The LM primordia appeared on the 8th day of incubation as a raised elevation carried on a deep median symmetrical sulcus (glottis primordium).
View Article and Find Full Text PDFEstrogenic-like compounds severely disturb germ cell formation in Japanese quail.
Biochem Biophys Res Commun
February 2025
Department of Biological Sciences, Faculty of Science, Hokkaido University, Kita 10 Nishi 8, Kita-ku, Sapporo, Hokkaido, 060-0810, Japan.
During avian germ cell formation, primordial germ cells (PGCs) differentiate into prospermatogonia in testicular seminiferous tubules or into oogonia in the ovarian cortex in late-stage embryos. Although estrogenic endocrine-disrupting chemicals (EDCs) have been suggested to affect the differential fate of avian germ cells, there is currently no established method to examine the effects of EDCs on the differentiation potential of germline cells due to large amount of unidentified proteins present in avian germ cells. Regarding reliable molecular probes for the detection of germ cells that differentiated from the PGCs of Japanese quail, the prospermatogonium and oogonium, respectively, integrin beta1 (ITGB1), insulin-like growth factor 2-binding protein 1 (IGF2BP1), and stimulated by retinoic acid 8 (STRA8) were identified as marker proteins by RNA-seq and liquid chromatography tandem mass spectrometry analyses.
View Article and Find Full Text PDFEffect of dietary supplementation of jujube fruit (Ziziphus jujuba) powder on performance, some biochemical parameters, and egg quality in quails (Coturnix coturnix japonica).
Pol J Vet Sci
June 2024
Elazıg Veterinary Control Institute, Republic of Turkey Ministry of Agriculture and Forestry, 23200, Elazig, Turkey.
This study aimed to evaluate the effects of different dietary supplementation levels with jujube fruit powder on the performance, biochemical parameters, and egg quality characteristics of laying quails. A total of 60 quails (45 days old) were randomly assigned to treatments with different levels of jujube fruit powder: a basal diet (control) and diets supplemented with 5 g/kg (T1), 10 g/kg (T2), with five replicates per treatment (20 quails/treatment and four quails/replicate). The differences between 1-15 and 16-30 days for feed intake (p<0.
View Article and Find Full Text PDFMolecular responses to clove and oregano essential oils are associated with reduced inflammation and improved gut barrier function in broiler chickens.
Poult Sci
December 2024
Animal Bioscience and Biotechnology Laboratory United States Department of Agriculture-Agricultural Research Service, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA. Electronic address:
In vitro tests were conducted to characterize the host-mediated responses of chickens to Clove Essential Oil (CEO) and Oregano Essential Oil (OEO). Chicken macrophage cells (CMCs), chicken intestinal epithelial cells (IECs), quail muscle cells (QMCs), and chicken embryonic muscle cells (EMCs) were utilized in these assays. EMCs were collected from the 13-day-old embryo during egg incubation and all cell lines were seeded at 2 × 10/mL in a 24-well plate.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!