AI Article Synopsis
Article Abstract
This report evaluates the potential of the antidepressant drug citalopram to induce homozygotization of genes previously present in a heterozygous condition, by homologous recombination. In order to address this question, a heterozygous diploid strain of the filamentous fungus Aspergillus nidulans and the homozygotization assay were utilized. Non-cytotoxic concentrations of citalopram (50, 75 and 100 μmol/L) showed a strong recombinogenic effect in A. nidulans, inducing homozygosis of the diploid strain's nutritional markers. The genetic markers exhibited homozygotization index (HI) rates higher than 2.0 and significantly different from HI control ones. Since citalopram has been previously characterized as a DNA synthesis inhibitor, the recombinogenic potential of this antidepressant in A. nidulans may be associated with the recombinational repair of citalopram-induced DNA strand breaks.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1258/ebm.2010.010159 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Overexpression of the RNA-binding protein NrdA affects global gene expression and secondary metabolism in species.
mSphere
January 2025
United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima, Japan.
RNA-binding protein Nrd1 plays a role in RNA polymerase II transcription termination. In this study, we showed that the orthologous NrdA is important in global mRNA expression and secondary metabolism in species. We constructed an conditional expression strain using the Tet-On system in mut.
View Article and Find Full Text PDFIsolation and characterization of native fungal strains for the bioremediation of BTEX pollutants.
Fungal Biol
February 2025
Department of Animal Biology, Faculty of Natural Sciences University of Tabriz, Tabriz, Iran; Microbial Biotechnology Research Group, Faculty of Natural Sciences University of Tabriz, Tabriz, Iran. Electronic address:
Benzene, toluene, ethylbenzene, and xylene (BTEX) exposure is known to be carcinogenic and neurotoxic chemicals to humans. This study investigates the potential of fungal native strains for the bioremediation of BTEX compounds. Fungal isolates were obtained from BTEX-enriched soil, and their ability to degrade these pollutants was evaluated.
View Article and Find Full Text PDFTranscription Factors ClrB and XlnR and Their Effect on the Transcription of Cellulase Genes in the Filamentous Fungus .
Int J Mol Sci
December 2024
Federal Research Centre "Fundamentals of Biotechnology", Russian Academy of Sciences, Moscow 119071, Russia.
The filamentous fungus (anamorph ) has been shown to be an efficient producer of secreted cellulases, used in biorefinery processes. Understanding the mechanisms of regulation of cellulase gene expression in the fungus is a current task in industrial biotechnology, since it allows for targeted changes in the composition of the complex secreted by the fungus. Expression of cellulase genes in fungi is regulated mainly at the level of transcription via pathway-specific transcription factors (TF), the majority of which belong to the Zn(II)2Cys6 family of zinc binuclear cluster proteins.
View Article and Find Full Text PDFUnlocking Testosterone Production by Biotransformation: Engineering a Fungal Model of Strain Deficient in Steroid 11α-Hydroxylase Activity and Expressing 17β-Hydroxysteroid Dehydrogenase Enzyme as Proof of Concept.
Biomolecules
November 2024
Área de Bioquímica y Biología Molecular, Departamento de Biología Molecular, Universidad de León, 24007 León, Spain.
Testosterone holds significant medical and economic importance, with the global market for testosterone replacement therapies valued at approximately USD 1.9 billion in 2023. This hormone is essential for the development and maintenance of male sexual characteristics as well as bone and muscle health.
View Article and Find Full Text PDFNidustrin A, cysteine-retained emestrin with a unique 18-membered macrocyclic lactone from the endophytic fungus Aspergillus nidulans.
Bioorg Chem
February 2025
Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China. Electronic address:
Nidustrin A (1), the first cysteine-retained emestrin featuring a unique sulfur-containing 18-membered macrocyclic lactone, along with four biogenetically related compounds (2-5), and one known analogue secoemestrin C (6), were isolated from the large-scale culture of Aspergillus nidulans, an endophytic fungus derived from the Whitmania pigra. Compounds 2 and 3 represent the second examples of noremestrin besides the previously reported noremestrin A, and the single crystal X-ray diffraction analysis of compound 2 provided solid evidence for the intriguing skeleton of noremestrin. Their structures were determined by extensive spectroscopic data, electronic circular dichroism calculations, and single-crystal X-ray diffraction.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!