Identification of plant tissues from the gut of Phlebotomus papatasi by DNA analysis.

Acta Trop

Department of Microbiology and Molecular genetics, Institute for Medical Research Israel-Canada, Kuvin Centre for the Study of Infectious and Tropical Diseases, Faculty of Medicine, Hebrew University, Jerusalem 91120, Israel.

Published: January 2011

AI Article Synopsis

  • Sugar and cellulose assays can indicate if insects like Phlebotomus papatasi have fed on sugars or plant tissues, but can't specify the plant species.
  • In a study conducted in the Lower Jordan Valley, researchers combined DNA analysis with these assays to better understand what these sand flies are feeding on.
  • Findings revealed that 37% of the flies contained sugar, 45% showed cellulose, and 41% had detectable plant DNA, with the DNA primarily matching the plant Suaeda asphaltica.

Article Abstract

Sugar and cellulose assays can determine if an insect has fed on sugar and plant tissue but they cannot identify the species of source plant. In this study, we used DNA analysis together with traditional cellulose and sugar assays to examine the plant feeding habits of wild Phlebotomus papatasi during the dry season in the Lower Jordan Valley, Israel. About 37% of the sand flies were positive for sugar, 45% were positive for cellulose in the gut, and 41% contained amplifiable plant DNA. All of the plant DNA fragments sequenced from both males and females were identified as Suaeda asphaltica.

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Source
http://dx.doi.org/10.1016/j.actatropica.2010.08.019DOI Listing

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