Analysis of Amaryllidaceae alkaloids from Zephyranthes robusta by GC-MS and their cholinesterase activity.

Nat Prod Commun

Department of Pharmaceutical Botany and Ecology, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.

Published: August 2010

From the bulbs of Zephyranthes robusta Baker (Amaryllidaceae), seven known compounds, belonging to four structural types of Amaryllidaceae alkaloids, were identified and quantified by GC-MS. The alkaloid extract from the bulbs showed promising acetylcholinesterase and butyrylcholinesterase inhibitory activities against HuAChE (IC50 = 35.9 +/- 3.5 microg/mL) and HuBuChE (IC50 = 190.9 +/- 8.2 microg/mL).

Download full-text PDF

Source

Publication Analysis

Top Keywords

amaryllidaceae alkaloids
8
zephyranthes robusta
8
+/- microg/ml
8
analysis amaryllidaceae
4
alkaloids zephyranthes
4
robusta gc-ms
4
gc-ms cholinesterase
4
cholinesterase activity
4
activity bulbs
4
bulbs zephyranthes
4

Similar Publications

Genetic variation in the α5 nicotinic acetylcholine receptor (nAChR) subunit of mice results in behavioral deficits linked to the prefrontal cortex (PFC). rs16969968 is the primary Single Nucleotide Polymorphism (SNP) in CHRNA5 strongly associated with nicotine dependence and schizophrenia in humans. We performed single cell-electrophysiology combined with morphological reconstructions on layer 6 (L6) excitatory neurons in the medial PFC (mPFC) of wild type (WT) rats, rats carrying the human coding polymorphism rs16969968 in Chrna5 and α5 knockout (KO) rats.

View Article and Find Full Text PDF

Narciclasine attenuates sepsis-associated acute kidney injury through the ESR1/S100A11 axis.

Funct Integr Genomics

January 2025

Department of Emergency and Critical Care Medicine, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210028, People's Republic of China.

Narciclasine (Ncs) was effective in sepsis management due to its antioxidant properties. The present study dissected the protective effects of Ncs against sepsis-associated acute kidney injury (SA-AKI) and the molecular mechanisms. The SA-AKI mice were developed using cecum ligation and puncture and pretreated with Ncs and adenoviruses.

View Article and Find Full Text PDF

The genus (Amaryllidaceae) currently contains 25 plant species naturally occurring in Europe and the Middle East region. These perennial bulbous plants possess well-known medicinal and ornamental qualities. Alkaloid diversity is their most distinctive phytochemical feature.

View Article and Find Full Text PDF

Forty-two Amaryllidaceae alkaloids, including eleven previously undescribed alkaloids, crinasiaticines C-M, and three undescribed naturally occurring alkaloids, (+)-dihydroepivittatine, (+)-dihydrovittatine and (+)-dihydrohamayne, were isolated from the leaves of Crinum asiaticum L. var. asiaticum.

View Article and Find Full Text PDF

Lycorine esters exert anti-HCoV-OC43 effect through reversibly acylating cysteine residue in the nsp 12 NiRAN domain.

Bioorg Chem

January 2025

State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China. Electronic address:

By introducing ester warheads into the hydroxyl groups in lycorine (1), three types of lycorine mono-ester or di-ester analogues were synthesized and evaluated for their antiviral activities against HCoV-OC43. Most of them showed higher selective indexes (SI) than 1, up to nearly 14 times. Using compound 6b as a probe, we firstly demonstrated that lycorine esters directly targeted nidovirus RdRp-associated nucleotidyltransferase (NiRAN) domain in the non-structural protein 12 (nsp 12) by reversibly acylating Cys12 to induce the shrink of NiRAN pocket and block the viral replication, different from the known RdRp inhibitors.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!